摘要
目的:探讨染料木黄酮对氨所致星形胶质细胞NF-κB活化的影响及其机制。方法:以氯化铵刺激原代培养的星形胶质细胞,建立高血氨模型,Western blot检测星形胶质细胞中ERK、Akt及核因子κB(NF-κB)的活化。结果:AG1478及染料木黄酮可显著抑制氨诱导的星形胶质细胞ERK及Akt的活化。LY294002、染料木黄酮及AG1478可显著抑制氨诱导的星形胶质细胞NF-κB核转位。结论:染料木黄酮可显著抑制氨诱导的星形胶质细胞ERK活化和Akt介导的NF-κB活化,这可能是该天然物质抑制星形胶质细胞水肿的重要机制。
AIM： To explore the effect of genistein on ammonia-induced nuclear factor-κB（NF-κB） activation and the underlying mechanism. METHODS： Primary astrocyte cultures were prepared and challenged with NH_4Cl to establish a hyperammonemic model. The activation of ERK,Akt and NF-κB was examined by Western blot. RESULTS：AG1478 and genistein significantly inhibited ammonia-induced activation of ERK and Akt. Ammonia-induced NF-κB nuclear translocation was significantly inhibited by the pretreatment of LY294002,genistein and AG1478. CONCLUSION：Genistein significantly inhibited ammonia-induced ERK activation and Akt-mediated NF-κB activation,which might represent the important mechanism by which this naturally occurring substance exerts its swelling-inhibiting effect.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2017年第9期1598-1601,共4页
Chinese Journal of Pathophysiology
基金
辽宁省自然科学基金资助项目(No.2015020360)
辽宁医学院校长基金-奥鸿博泽基金(No.XZJJ20140111)
辽宁医学院校长基金-奥鸿博泽大学生科技创新基金(No.2015D20)
2015年辽宁省大学生创新创业训练计划项目(乙类)(No.201510160000035)
