摘要
[目的]研究适宜于北柴胡新品种川北柴1号的组织培养快繁技术。[方法]以川北柴1号茎节为材料,分别以MS和1/2MS为基本培养基,通过不同的激素种类和浓度,分析诱导川北柴1号丛生芽和不定根生长的最佳培养基配方。[结果]在MS培养基中,1.0mg/L的6-BA,0.5mg/L的KT,0.2mg/L的NAA和1.0mg/L的IAA在显著促进不定芽增殖的同时,还能最大化促进不定芽生长;在1/2MS培养基中,0.1mg/L浓度NAA,1.0mg/L浓度IBA和1.0mg/L浓度IAA对柴胡不定根生长促进作用最佳。[结论]本研究对川北柴1号生产推广制种具有重大意义。
[Objective]identify a protocol of tissue culture for commercial variety of Chuanbeichai No.1 in Bupleurum chinense DC.[Method]the stem of Chuanbeichai No.1 was used to induce shoots in MS medium, and subsequent induce root in 1/2 MS medium.The effect of growth regulators with different concentrations was also analyzed. [Results]the adding of 1.0 mg/L 6-BA, 0.5 mg/L KT, 0.2 mg/L NAA,or 1.0 mg/L IAA in MS medium resulted in the best multiplication. The adding of 0.1 mg/L NAA,1.0 mg/L IBA, or 1.0 mg/L IAA in 1/2 MS medium resulted in the best root formation. [Conclusion] this micropropagation is very important to large scale of seed production for Chuan-beichai No. 1.
出处
《种子》
北大核心
2017年第9期27-29,34,共4页
Seed
基金
国家自然科学基金(81603223)
四川省"十三五"科技育种攻关(2016 NYZ 0036-4-2)
四川省科技扶贫专项(2016FP0071
2016NFP 0055)
关键词
北柴胡
川北柴1号
生长激素
组织培养
Bupleurum chinense DC
chuanbeichai No.l
growth regulators
tissue culture
