摘要
目的 探究IFN-γ对IL-33诱导的小鼠急性过敏性气道炎症中2型固有淋巴细胞（ILC2s）的抑制作用.方法 将24只6～8周的雌性C57BL/6小鼠随机分为4组：IL-33模型组、单独IFN-γ组、IL-33＋IFN-γ联合刺激组、PBS对照组.用IL-33成功构建小鼠支气管过敏性炎症模型,收集支气管肺泡灌洗液（BALF）和肺组织,使用流式细胞术对ILC2s和嗜酸性粒细胞进行分析.酶联免疫吸附法（ELISA）测定肺匀浆和BALF上清中IL-5和IL-13的含量.Real-time PCR检测IL-5、IL-13和ST2 mRNA表达量的变化.肺组织切片进行HE和PAS染色观察病理变化.结果 与PBS对照组相比,IFN-γ组未见炎细胞浸润、杯状细胞增生及黏液分泌;ILC2s和嗜酸性粒细胞含量无差异（P〉0.05）;IL-5、IL-13水平差异无统计学意义（P〉0.05）;IL-5、IL-13、ST2 mRNA表达量差异无统计学意义（P〉0.05）.与PBS对照组相比,IL-33模型组有大量炎性细胞浸润,杯状细胞增生并分泌大量黏液;ILC2s和嗜酸性粒细胞含量明显增多,差异有统计学意义（P〈0.05）;IL-5、IL-13水平升高,差异有统计学意义（P〈0.05）;IL-5、IL-13、ST2 mRNA表达量增高,差异有统计学意义（P〈0.05）.与IL-33组相比,IFN-γ＋IL-33组炎细胞浸润减弱,杯状细胞增生受到抑制且黏液分泌量减少;IL-5、IL-13水平降低,差异有统计学意义（P〈0.05）;IL-5、IL-13、ST2 mRNA表达量降低,差异有统计学意义（P〈0.05）.结论 实验证实IFN-γ可抑制IL-33引起的嗜酸性粒细胞和ILC2s的增殖,减弱IL-5、IL-13的分泌,提示：IFN-γ对IL-33诱导的小鼠急性过敏性气道炎症有抑制作用.
Objective To investigate the inhibitory effect of IFN-γ on acute allergic airway inflammation induced by IL-33 in mice.Methods Twenty-four female C57BL/6 mice （6-8 weeks） were randomly divided into four groups： IL-33 model group, IFN-γ treatment group, IL-33＋IFN-γ treatment group and PBS control group.A mouse model of acute allergic airway inflammation was induced by IL-33.Samples of bronchial alveolar lavage fluid （BALF） and lung tissues were collected.Group 2 innate lymphoid cells （ILC2s） and eosinophils were analyzed by flow cytometry.Levels of IL-5 and IL-13 in the supernatants of lung homogenate and BALF were measured by ELISA.Expression of IL-5, IL-13 and ST2 at mRNA level was detected by real-time PCR.Pathological changes in lung tissues were observed following hematoxylin and eosin （HE） and periodic acid-Schiff （PAS） staining.Results Compared with the PBS control group, no infiltration with inflammatory cells, goblet cell hyperplasia or mucus secretion was observed in the IFN-γ group;the numbers of ILC2s and eosinophils were not affected by IFN-γ;the levels of IL-5 and IL-13 in the supernatants of BALF and lung homogenate, and the expression of IL-5, IL-13 and ST2 at mRNA level in lung tissues were not significantly changed by IFN-γ （P〉0.05）.Compared with the PBS control group, massive infiltration with inflammatory cells, excessive mucus secretion, increased numbers of ILC2s and eosinophils, up-regulated levels of IL-5 and IL-13 in the supernatants of BALF and lung homogenate, and enhanced expression of IL-5, IL-13 and ST2 at mRNA level in lung tissues were detected in the IL-33 model group （P〈0.05）.Compared with the IL-33 model group, the combined treatment with IL-33 and IFN-γ significantly alleviated inflammatory cell infiltration, inhibited mucus secretion, reduced the numbers of ILC2s and eosinophils, down-regulated the levels of IL-5 and IL-13 in the supernatants of BALF and lung homogenate, and suppressed the expression of IL-5, IL-13 and ST2 at mRNA in l
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2017年第8期573-579,共7页
Chinese Journal of Microbiology and Immunology
