摘要
目的研究丹参通脉方对人主动脉血管内皮细胞(human vascular endothelial cell,HVEC)同型半胱氨酸(HCY)诱导模型内质网应激(ERS)偶联凋亡及炎症调节作用的影响,探讨丹参通脉方抗动脉粥样硬化的详细机制。方法应用HCY制作HVEC凋亡模型,丹参通脉方干预24h后,采取Annexin V-FITC/PI法检测细胞凋亡,免疫荧光检测ERS通路中BIP蛋白,实时荧光定量PCR法检测ERS通路中BIP、Chop的m RNA表达,同时使用ELISA法检测HVEC培养上清液中炎症因子表达。结果丹参通脉方抑制HVEC凋亡,与空白对照组及HCY模型组相比有统计学意义(P<0.05);丹参通脉方同时可抑制BIP、Chop细胞内m RNA的表达,通脉方组与空白组及模型组相比有统计学意义(P<0.05);丹参通脉方可抑制IL-6,IL-8及MCP-1炎症因子的表达(P<0.01)。结论丹参通脉方可抑制HCY诱导的HVEC凋亡,减轻HVEC炎症反应,其具体机制可能与抑制ERS有关。
Objective To study the Anti-apoptotic and anti-inflammatory effects of Danshentongmai on human vascular endothelial cells（ HVEC） via endoplasmic reticulum stress（ ERS） and its mechanism of anti-atherosclerosis. Methods Established in vitro model of apoptotic HVEC by homosysteine（ HCY）,using Danshentongmai as an interfering drug,detected apoptosis rate by Annexin V-FITC/PI methods,and quantified the amount of BIP by immuno-fluorescence and quantified the amount of BIP,Chop by Real-time reverse transcript PCR. Results Danshentongmai inhibited the apoptosis of HVEC compared with the blank and the model group（ P〈0. 05）,Danshentongmai attenuated the protein content and the gene expression of ERS markers compared with the blank and the model group too（ P〈0. 05）,and Danshentongmai inhibited IL-6,IL-8 and MCP-1 expressions（ P〈0. 01）. Conclusion Danshentongmai could inhibit the apoptosis and inflammatory response in HVEC,the mechanism may related to ERS.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2017年第8期1808-1811,共4页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.81273949)
河南省基础与前沿技术研究计划项目(No.152300410110)
河南省高等学校重点科研项目(16A310027)
