摘要
目的为建立有效的动物模型,更好研究激素性股骨头坏死的发病机制,科学阐明中药方剂治疗该疾病的分子作用机制。方法取45只2月龄雄性C57BL/6小鼠随机平均分为对照组、传统组、改良组。传统组给予脂多糖联合甲强龙激素造模,改良组小鼠则在传统组基础上调整饲料槽的高度,迫使小鼠主动站立取食,并予跑步机上跑步2 h/d;对照组给予等量的生理盐水。定时观察小鼠一般状况,分别于造模前、造模后第4周、第8周抽血检测血液中三酰甘油的含量,并于造模后8周测量小鼠股骨头骨密度,次日处死取双侧股骨头,体视显微镜下观察股骨头外观及色泽。采用苏木精-伊红染色法(HE)及阿尔新蓝染色(ABH)等技术观察小鼠股骨头病理变化。结果传统组和改良组小鼠精神状态明显差于对照组,且改良组差于传统组(P<0.05);传统组和改良组血液中三酰甘油的含量高于对照组,改良组高于传统组;骨密度检测显示,传统组和改良组骨密度低于对照组,且改良组低于传统组(P<0.05);股骨头形态学观察发现改良组股骨头软骨下出血坏死的面积较传统组严重,HE、ABH结果显示,传统组有9只出现(空骨陷窝数量增多,骨小梁变细甚至断裂)等骨坏死表现,造模成功率达60%,改良组有11只出现(空骨陷窝数量增多,骨小梁变细甚至断裂)股骨头坏死的表现,造模成功率高达73.3%(P<0.05)。结论传统造模方法和改良造模方法皆可造模成功,但改良造模方法具有更高成模率。
Objective： To establish an effective animal model to study the pathogenesis of steroid induced osteonecrosis of the femoral head,and to elucidate the molecular mechanism of the treatment of the disease. Methods： 45 male C57BL/6 mice of two months′ old were randomly divided into three groups： the control group（A group） and the traditional group（B group） and the modified group（C group）. The traditional group was treated with lipopolysaccharide combined with methylprednisolone. In the modified group,the height of the feed tank was adjusted on the basis of the traditional group,forcing the mice to stand actively for feeding,and running on the treadmill,2 h/d. A group was given the same dose of saline,regularly observing the general condition of mice. Respectively before and four and eight weeks after modeling,three glycerol content in the blood was detected. The femoral head density was measured 8 weeks after modeling,and the mice was sacrificed and the bilateral femoral head was taken the next day. The appearance and color of the femoral head were observed under a stereo micro-scope. Hematoxylin and eosin（HE） staining and Alcian blue staining（ABH） were used to evaluate the pathological changes of mouse femoral head. Results： Mental state of B and C groups was worse than that of A group,and C group was worse than B group（P 0.05）. In the traditional group and the modified group,the content of three glycerol in the blood was higher than that in the control group,and the modified group was higher than the traditional group. Bone mineral density test showed that bone density in the traditional group and the modified group was lower than that in the control group,and the modified group was lower than that of the traditional group（P 0.05）. The morphological observation of femoral head showed that the area of subchondral hemorrhage and necrosis in the femoral head of the modified group was more serious than that in the traditional group. Hematoxylin-eosin（HE） staining and Alcian blu
出处
《中国中医急症》
2017年第9期1525-1528,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
国家自然科学基金项目(81273770,8157150451)
浙江省自然科学基金项目(LY15H270012,Q17H270023)
浙江省中医药科技计划项目(2017ZB026)
关键词
激素性股骨头坏死
动物模型
甲强龙
脂多糖
跑步机
Steroid induced osteonecrosis of the femoral head
Animal model
Methylprednisolone
Lipopolysaccharide
Treadmill
