摘要
【目的】探讨内热针治疗对第三腰椎横突综合征模型大鼠损伤组织炎症反应的影响。【方法】将90只SD大鼠随机分成6组:正常组、模型组、药物组、内热针组、普通针刺组和温针灸组,每组15只。采用埋置明胶海绵的方法制作第三腰椎横突综合征模型,2周后对相应组别大鼠进行治疗。于治疗后第0、7、14、28天从大鼠颈静脉取血,采用酶联免疫吸附反应(ELISA)法检测血液中炎性细胞因子如肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β和IL-6的含量;于治疗后第28天处死大鼠,取第三腰椎横突周围肌肉组织,采用HE染色观察肌肉组织病理学变化,采用实时定量逆转录—聚合酶链反应(RT-q PCR)检测肌肉组织中炎性细胞因子TNF-α、IL-1β和IL-6的mRNA表达情况。【结果】造模后的大鼠血清中TNF-α、IL-1β和IL-6含量较正常组显著升高(P<0.05);治疗后,各治疗组大鼠血清中上述炎性细胞因子含量较模型组显著降低(P<0.05),且内热针组最为明显(P<0.05)。治疗后,各治疗组大鼠损伤肌肉组织TNF-α、IL-1β和IL-6的mRNA水平较模型组显著降低(P<0.05),且内热针组表达水平最低。内热针组大鼠肌肉组织苏木素—伊红(HE)染色结果与其他治疗组比较,炎症反应更轻,肌纤维结构更完整。【结论】内热针治疗可通过降低TNF-α、IL-1β和IL-6等炎性细胞因子的表达,改善局部炎症反应,促进组织损伤的修复。
Objective To observe the effect of heat needle treatment on the inflammatory reaction in the damaged tissues of rats with the third lumbar transverse process syndrome. Methods Ninety Sprague-Dawley rats were randomly divided into 6 groups,namely normal group,model group,drug group,heat needle group,conventional acupuncture group and warming acup-moxibustion group, 15 rats in each group. The rat model of the third lumbar syndrome was established by embedding gelatin sponge. Two weeks later,the rats in various groups were given corresponding treatment respectively. Before treatment and on the 7th, 14th and 28th day of treatment,the blood samples were collected from jugular vein,and then the serum inflammatory cytokines of tumor necrosis factor alpha（TNF-α）,interleukin（IL）-1β and IL-6 were detected by enzyme-linked immunosorbent assay（ELISA）. On the 28th day of treatment,all of the rats were sacrificed,the expression levels of TNF-α,IL-1β and IL-6 mRNA in the muscle tissue were detected by reverse transcription quantitative real-time polymerase chain reaction（RT-q PCR）,and the pathological changes in local muscle tissue were observed by HE staining method. Results The serum contents of the inflammatory cytokines of TNF-α, IL-1β and IL-6 in the model group and treatment groups after modeling were significantly higher than those in the normal group（P〈 0.05）.After treatment, the contents of TNF-α, IL-1β and IL-6 in various treatment groups were decreased significantly as compared with those in the model group,and the decrease was most obvious in the heat needlegroup. The expression levels of TNF-α,IL-1β and IL-6 mRNA in muscle tissues of drug group,heat needle group,conventional acupuncture group and warming acup-moxibustion group were lower than those of the model group（P〈 0.05）,and the heat needle group had the lowest levels. HE staining results showed that heat needle group had milder inflammatory reaction and better muscle fiber structure than the model group. Conclusion Heat n
作者
骆小娟
张照庆
尹晶
徐芸
段轶轩
夏杨
居诗如
LUO Xiao-Juan ZHANG Zhao-Qing YIN Jing XU Yun DUAN Yi-Xuan XIA Yang JU Shi-Ru(Dept. of Rehabilitation, Wuhan Third Hospital, Wuhan 430061 Hubei, China)
出处
《广州中医药大学学报》
CAS
2017年第5期703-707,共5页
Journal of Guangzhou University of Traditional Chinese Medicine
