摘要
大豆花叶病毒(soybean mosaic virus,SMV)是一种株系复杂且不断变化、并对大豆的产量和质量造成恶劣影响的病毒,其严重的致病性受到学者的普遍关注。大豆花叶病毒侵染性克隆技术是研究其致病机理的行之有效的手段。该技术通过病毒侵染获得侵染性cDNA克隆,进一步分析引起症状的相应基因在植株生长过程中的作用机制,由此为SMV相关领域的研究奠定理论基础。本文就大豆花叶病毒侵染性克隆的构建策略和影响因素,以及其应用状况作一综述,以期为大豆花叶病毒的防治提供新的借鉴和思路。
Soybean mosaic virus （SMV） is a complex and ever-changing virus that has a negative effect on tbe yield and qual- ity of soybeans. Its serious pathogenicity is generally concerned by scholars associated with the field of soybean research. The SMV is mainly infected with soybean aphids. The research teehnique of soybean mosaic virus infection cloning was effective and widely adopted to study its pathogenic mechanism. This technique can further analyze the mechanism of the genes that cause the corresponding symptoms in the process of plant growth through the obtained infectious cDNA clones. At the same time, prepared monoclonal antibody can be used as the development of soybean mosaic virus detection kit probe material. As a basis for the study of SMV related fields, based on the relationship between soybean mosaic virus disease and SMV infection cloning technique. This paper briefly summarizes the construction strategy and influencing factors of SMV infectious clone, and summarizes the application of this technology in recent years, to provide new reference and thought for the control of soy- bean mosaic virus.
出处
《大豆科学》
CAS
CSCD
北大核心
2017年第5期808-812,共5页
Soybean Science
基金
东北农业大学大学生创新基金(201610224147)
哈尔滨市应用技术研究与开发项目(2014AB6BN036)
中央引导地方科技发展专项(ZY16C06)
关键词
大豆
大豆花叶病毒
侵染性克隆
Soybean
Soybean mosaic virus
Infectious clones
