摘要
目的研究天麻素对模拟高原缺氧大鼠动脉血气及脑组织损伤的影响。方法成年健康雄性Wistar大鼠60只,随机分为常氧模型(N)组,缺氧模型(M)组,红景天(RC)对照组,天麻素低剂量(GAS-L)组,天麻素中剂量(GAS-M)组,天麻素高剂量(GAS-H)组,每组10只。每日定时灌胃给药,连续7d。采用低压氧舱模拟海拔8000m高原环境,缺氧12h后,测定各组大鼠动脉血气,观察大鼠脑组织病理变化,并测定脑组织相关生化指标水平。结果与N组比较,M组大鼠氧分压(PO_2)、血氧饱和度(SO_2)、氧合指数(PO_2/Fi O_2)、钠离子(Na+)、实际碳酸氢根(HCO3–)明显下降(P<0.01),乳酸(Lac)、血红蛋白(Hb)浓度明显上升(P<0.01);大鼠脑组织出现病理性损伤;脑组织中丙二醛(MDA)、过氧化氢(H_2O_2)含量明显升高(P<0.01),谷胱甘肽(GSH)含量、谷胱甘肽过氧化物酶(GSH-Px)活性明显降低(P<0.01)。与M组比较,GAS-L组PO_2、SO_2、PO_2/FiO_2明显升高(P<0.05,P<0.01);GAS三个剂量组Na^+、HCO_3~–均明显升高(P<0.05,P<0.01);GAS-L及GAS-H组Lac含量明显降低(P<0.01,P<0.05);GAS-H组Hb明显升高(P<0.01)。RC及GAS组脑组织病理损伤较M组轻。与M组相比,GAS三个剂量组脑组织MDA明显降低(P<0.05,P<0.01),H_2O_2有降低趋势,但差异无统计学意义(P>0.05);GSH含量及GSH-Px活性均明显升高(P<0.05,P<0.01),但无明显剂量依赖性。结论天麻素能够对模拟高原缺氧大鼠动脉血气及脑组织损伤起到保护作用。
Objective To study the effect of gastrodin on arterial blood gas and brain injury of rats under simulated high altitude hypoxia environment. Methods A total of 60 adult healthy male Wistar rats were randomly divided into normal （N） group, hypoxia model （M） group, rhodiola crenulata （RC） group, low dose of gastrodin （GAS-L） group, medium dose of gastrodin （GAS-M） group and high dose of gastrodin （GAS-H） group （10 for each group）. The intragastric administration on rats was continued for 7 days timely in each day. Under simulated 8000m altitude using low pressure oxygen cabin, the arterial blood gas of each group were tested, pathological changes of brain tissues were observed and related indexes of brain were detected after 12h hypoxia. Results Comparing with group N, the blood oxygen partial pressure （PO2）, value of blood oxygen saturation （SO2）, oxygenation index （PO2/FIO2）, Na＋ concentration （Na＋）, actual bicarbonate radical （HCO3–） significantly decreased （P〈0.01）, lactic acid （Lac）, hemoglobin concentration （Hb） significantly increased （P〈0.01） and pathological damage was inflicted in group M; and contents of malondialdehyde （MDA）, hydrogen peroxide （H2O2） in brain tissue significantly increased （P〈0.01）, content of glutathione（GSH） and activity of glutathione peroxidase （GSH-Px） in brain tissue significantly decreased （P〈0.01） in group M. Compared with group M, PO2, SO2 and PO2/FIO2 significantly increased （P〈0.01, P〈0.05） in group GAS-L; Na＋ and HCO3– significantly increased （P〈0.01, P〈0.05） in three dose groups of GAS; Lac significantly decreased （P〈0.01, P〈0.05） in group GAS-L and GAS-H. Hb significantly increased （P〈0.01） in group GAS-H, a rising trend appeared in group GAS-L but with no statistical significance. Damages of brain tissue were alleviated in group RC and three dose groups of GAS comparing with group M. Compared with group M, MDA significantly decreased （P
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2017年第8期728-732,共5页
Medical Journal of Chinese People's Liberation Army
基金
军队中医药科研专项课题(10ZYZ117)
湖北省自然科学基金(2015CFB301)
武汉市科技计划项目(2017060201010218)~~
关键词
天麻素
缺氧
脑
高原病
gastrodin
hypoxia
brain
altitude sickness
