摘要
在干旱胁迫下,橡胶树过氧化物酶活性显著增加。为了研究干旱响应的过氧化物酶基因功能,从橡胶树中克隆了HbPRX53全长DNA。生物信息学分析结果表明,HbPRX53含有334个氨基酸和1个植物过氧化物酶结构域。聚类分析结果发现,其与拟南芥过氧化物酶At PRX53最相近。HbPRX53在橡胶树树皮、叶片、胶乳和花中均有表达,但在叶片中表达量最高,白粉菌侵染、机械伤害和干旱显著上调叶片中HbPRX53的表达。此外,吲哚乙酸、脱落酸、水杨酸乙烯、过氧化氢和茉莉酸处理也会上调HbPRX53表达。这说明HbPRX53与橡胶树对生物和非生物胁迫的响应密切相关。
Peroxidase activity significantly increased under drought stress in rubber tree. To identify the functions of peroxidase genes responsive to drought stress, the full-length cDNA of HbPRX53 was isolated from rubber tree. Bioinformatics analysis showed that HbPRX53 contained 334 amino acid residues and a plant peroxidase-like superfamily domain. Phylogenetic analysis with Arabidopsis Class III peroxidases revealed that HbPRX53 shared high identities with AtPRX53. Although HbPRX53 was expressed in bark, leaf, latex and flower, it was preferentially expressed in leaf in rubber tree. HbPRX53 expression was significantly upregulated in leaves by powdery mildew infection, mechanical wounding and drought stress. Moreover, indole acetic acid, abscisic acid, salicylic acid ethylene, H202 and methyl jasmonate treatments also led to marked accumulation of HbPRX53 transcripts in leaves. These results indicated HbPRX53 is a key factor in both biotic and abiotic stress responses in rubber tree.
出处
《广东农业科学》
CAS
2017年第6期63-70,F0002,共9页
Guangdong Agricultural Sciences
基金
海南省自然科学基金(20153151)
国家现代农业产业技术体系建设专项(CARS-34-ZP1)
中国热带农业科学院橡胶研究所基本科研业务费专项(1630022015013)
