摘要
目的探讨瘦素与瘦素受体在子宫内膜异位组织中的表达。方法选取32例子宫内膜异位症患者作为内异组,并选取同期行输卵管结扎术的32例妇女作为对照组,分别采取两组患者的血清、腹腔液(PF)和腹膜组织(PI)。内异组患者切除子宫内膜异位囊肿,切取囊肿组织(OE),并吸取囊肿液(EF)。采用Western blot法测定PI和OE瘦素及瘦素受体表达水平。采用ELISA法测定血清、PF及EF中的瘦素水平。结果两组PI中瘦素和瘦素受体表达水平比较,差异均无统计学意义(均P>0.05),而内异组OE中瘦素和瘦素受体表达水平均较PI中瘦素及瘦素受体表达水平明显增高(均P<0.05)。两组血清及PF中瘦素水平比较,差异均无统计学意义(均P>0.05)。但内异组EF中瘦素水平明显高于血清及PF中瘦素水平,差异均有统计学意义(均P<0.01)。内异组患者OE与EF、PI与PF、OE与PF的瘦素表达水平均呈正相关(均P<0.05)。OE中瘦素与瘦素受体、EF瘦素与OE瘦素受体、PI中瘦素与瘦素受体、PF瘦素与PI瘦素受体均呈正相关(均P<0.05),而PF瘦素与OE瘦素受体表达水平无相关性(P>0.05)。结论瘦素通过与瘦素受体之间的相互调节作用在子宫内膜异位囊肿的发病过程中扮演了非常重要的角色。
Objective To investigate the expression of leptin and leptin receptor in endometriosis.Methods Thirty-two patients with endometriosis （EM group） and 32 women undergoing tubal ligation （control group） were enrolled in the study.The serum,peritoneal fluid （PF）,peritoneal biopsy （PI） samples were collected in both groups,the endometriosis cyst （OE） and aspiration of endometriosis cysts （EF） taken duting endometriosis resection were collected in EM group.The expression levels of leptin and leptin receptor in PI and OE were measured by Western blot,the levels of leptin in serum,PF and EF were measured by human leptin ELISA kit.Results The levels of leptin and leptin receptor in PI were not statistically significant between two groups （all P 〉0.05）.In EM group the expression of leptin and leptin receptor in OE were significantly higher than that in Pl（all P〈 0.05）.There were no significant difference in serum and PF leptin levels between the two groups（all P 〉0.05）.But in EM group the levels of leptin in EF were significantly higher than those in serum and PF （all P〈0.01）.In EM group there were positive correlations in expression levies of leptin and leptin receptor between OE and EF,PI and PF,OE and PF（all P〈0.05）,there were positive correlations between leptin and leptin receptor in OE,between leptin in EF and leptin receptor in OE,between leptin and leptin receptor in PI,between leptin in PF and leptin receptor in Pl（all P〈0.05）,while there was no correlation between leptin in PF and leptin receptor in OE （P 〉0.05）.Conclusion The interaction of leptin and leptin receptor may play an important role in the pathogenesis of endometriosis.
出处
《浙江医学》
CAS
2017年第13期1077-1079,1083,共4页
Zhejiang Medical Journal
