摘要
目的:研究番茄红素(lycopene,LP)对人非小细胞肺癌A549细胞增殖和凋亡的影响并探讨其机制。方法:体外培养并取对数生长期人非小细胞肺癌A549细胞,分别给予不同浓度的LP(2.5、5、10、20μg/ml)和顺铂(40μg/ml)进行干预,48 h后采用四甲基偶氮唑盐(MTT)比色法测定吸光度值并计算细胞增殖抑制率,流式细胞术(FCM)检测细胞周期和细胞凋亡状况,逆转录PCR法(RT-PCR)检测细胞中凋亡相关基因(Bax mRNA、bcl-2 mRNA)表达,免疫印迹法(Western blot)检测凋亡相关蛋白(caspase-3)表达。结果:与空白对照组比较,LP能够剂量依赖性地提高人非小细胞肺癌A549细胞增殖抑制率,延长细胞周期中G0/G1期并缩短G2/M期,提高A549细胞凋亡率(AI),上调Bax mRNA表达、下调bcl-2 mRNA表达,提高Bax/bcl-2比值,上调caspase-3蛋白表达。结论:LP具有抑制人非小细胞肺癌A549细胞增殖并促进其凋亡的作用,其机制可能与LP能够阻滞细胞周期并调节凋亡相关基因蛋白表达有关。
Objective：To investigate the effects and mechanism of lycopene （LP） on the proliferation and apoptosis of human non - small cell lung cancer A549 cell. Methods ： The human non - small cell lung cancer A549 cells in logarithmic growth phase were treated with lycopene （2.5,5,10,20 μg/ml） and cisplatin （40 μg/ml）. The cellular growth inhibition rate was tested by MTT. Cell cycle and apoptosis rates were analyzed by flow cytometry （FCM）. The expression of apoptosis -related genes （Bax mRNA and bcl -2 mRNA） were detected by RT- PCR, and the expres sion of caspase - 3 protein was detected by Western blot. Results ： Compared with blank control group, the cellular growth inhibition rate of human non - small cell lung cancer A549 cell in LP treated groups were significantly in- creased. The cell cycle was arrested at G0/G1 phase,the apoptosis rate was significantly increased. The expression of Bax mRNA was significantly increased, while the expression of bcl -2 mRNA was significantly decreased, and the ratio of Bax/bcl - 2 was significantly increased. The expression of caspase - 3 protein was significantly increased. All of the effects of lycopene above were dose - dependent with a certain. Conclusion： LP can inhibit human HepG2 cell proliferation and promote its apoptosis, which perhaps related to its effects of altering the cell cycle and the expression of apoptosis - related genes and proteins.
出处
《现代肿瘤医学》
CAS
2017年第19期3067-3070,共4页
Journal of Modern Oncology
关键词
番茄红素
肺癌
A549细胞
增殖
凋亡
lycopene, lung cancer, A549 cell, proliferation, apoptosis
