摘要
目的采用超高效液相色谱-飞行时间质谱(UPLC-Q-TOF-MS)对不同产地虫草化学成分进行鉴定与比较分析。方法采用超高效液相-飞行时间质谱(UPLC-Q-TOF-MS)及多元统计分析技术对实验结果进行分析,并对差异化合物进行鉴定。结果对不同产地的虫草进行了成分鉴定,核苷类物质含量高。不同产地虫草药材在组分种类和相对量上存在着一定的差异。湖南、湖北、丽江的虫草含有其他成分,丽江虫草含有大量的虫草素,湖南虫草含有一些黄酮类物质。采用主成分分析(PCA)将不同产地药材分别聚成不同组别,其中西藏(那曲)、四川、青海之间聚集紧密,而凉山、湖北、湖南及丽江虫草散布在四周,差别非常显著。结论 UPLC-Q-TOF-MS结合多元统计分析方法可为虫草药材的质量评价提供依据。
OBJECTIVE To identify and analyze the components of Cordyceps from different origins by UPLC-Q-TOF-MS. METHODS Metabolomic research of Cordyceps were performed using UPLC-Q-TOF-MS and multivariate statistical analysis technique. RESULTS Nucleosides were accounted for a large proportion of the identified components from Cordyceps. There was significant metabolites difference among Cordyceps from different origins, especially those from Hunan, Hubei and Lijiang. Cordyceps from Lijiang contained more deoxyadenosine than others and several flavonoids were detected in the Hunan sample. The Cordyceps from different origins were clustered into different groups respectively by principal component analysis（PCA）. Samples from Tibet, Sichuang and Qinghai were clustered closely, while those from Liangshan, Hunan, Hubei and Lijiang were scattered around. CONCLUSION This study indicated that UPLC-Q-TOF-MS coupled with multivariate statistics could provide quality control of Cordyceps.
出处
《中国现代应用药学》
CAS
CSCD
2017年第8期1145-1149,共5页
Chinese Journal of Modern Applied Pharmacy
基金
四川省科技计划项目(2017HH0036)
