摘要
目的:研究谷氨酰胺营养支持对急性重症胰腺炎患者肠黏膜屏障功能及炎症反应程度的影响。方法:选择在我院就诊的重症急性胰腺炎患者作为研究对象并随机分为两组,对照组患者接受常规对症治疗及常规肠内营养干预,Gln组接受常规对症治疗及谷氨酰胺肠内营养干预。治疗前及治疗后,检测血清中肠黏膜屏障损伤标志分子、炎症介质的含量及外周血中炎症信号分子的表达量;治疗后,检测肠道菌群的数量。结果:治疗后,两组患者血清中LPS、DAO、HBD2、TNF-α、sTREM-1、IL-1β、IL-6的含量以及外周血单个核细胞中TLR4、NF-kB、MyD88、p38MAPK的mRNA表达量均显著低于治疗前且Gln组患者治疗后血清中LPS、DAO、HBD2、TNF-α、sTREM-1、IL-1β、IL-6的含量以及外周血单个核细胞中TLR4、NF-kB、MyD88、p38MAPK的mRNA表达量均显著低于对照组,乳酸杆菌、双歧杆菌、拟杆菌的数量均显著高于对照组,大肠杆菌、肠球菌的数量均显著低于对照组。结论:谷氨酰胺营养支持用于急性重症胰腺炎能够减轻肠黏膜屏障功能损伤、抑制炎症反应的激活。
Objective：To study the effect of glutamine nutrition support on the intestinal mucosal barrier function and inflammatory response in patients with severe acute pancreatitis.Methods：Patients with severe acute pancreatitis who were treated in Pengzhou People＇s Hospital were selected as the research subjects and randomly divided into two groups,control group received conventional symptomatic treatment and conventional enteral nutrition intervention,and Gln group received conventional symptomatic treatment and glutamine enteral nutrition intervention.The contents of intestinal mucosal barrier damage markers and inflammatory mediators in serum as well as the expression of inflammatory signaling molecules in peripheral blood were detected before and after treatment;the number of intestinal flora was detected after treatment.Results：After treatment,LPS,DAO,HBD2,TNF-α,sTREM-1,IL-1βand IL-6levels in serum as well as TLR4,NF-kB,MyD88 and p38MAPK mRNA expression in peripheral blood mononuclear cells of both groups of patients were significantly lower than those before treatment,LPS,DAO,HBD2,TNF-α,sTREM-1,IL-1βand IL-6levels in serum as well as TLR4,NF-kB,MyD88 and p38MAPK mRNA expression in peripheral blood mononuclear cells of Gln group after treatment were significantly lower than those of control group,and the numbers of lactobacillus,bifidobacterium and bacteroides were significantly higher than those of control group while the numbers of escherichia coli and enterococcus were significantly lower than those of control group.Conclusions：Glutamine nutrition support for severe acute pancreatitis can reduce the intestinal mucosal barrier function injury and inhibit the inflammatory response activation.
出处
《海南医学院学报》
CAS
2017年第14期1896-1899,共4页
Journal of Hainan Medical University
基金
四川省医学科研课题(S16049)~~
关键词
急性重症胰腺炎
谷氨酰胺
肠内营养
肠黏膜屏障
炎症反应
Severe acute pancreatitis
Glutamine
Enteral nutrition
Intestinal mucosal barrier
Inflammatory response
