摘要
目的比较分析国产和进口2种EIA-HIV试剂对无偿献血者HIV1/2初筛结果的差异,探究减少1遍酶免检测的可行性。方法收集嘉兴市2009-2014年无偿献血者2种EIA-HIV1/2初筛试验阳性结果和WB法确认试验的检测数据,分析初筛试验与确认试验结果之间的关系;分析研究初筛试验S/CO值和确认试验结果间的关联性。结果国产和进口试剂的HIV阳性检出率分别为9.58/万和12.43/万,确认阳性预测值分别11.84%和9.12%,两者比较无统计学意义(χ~2=1.11,P>0.05);国产和进口联合检出阳性率为1.37/万,确认阳性预测值为82.86%,与单一试剂检测结果比较,均有差异性(χ~2国产=94.04,P<0.05和χ~2进口=124.86,P<0.05);S/CO值≥6.0时,国产和进口阳性预测值分别为93.55%(29/31)和87.50%(28/32)。结论国产和进口试剂EIA-HIV1/2之间无差异性。
Objective To compare the differences of two kinds of EIA reagents for HIV-1/2 （Ab/Ag） screening results of voluntary blood donors,in addition to find out the feasibility of reducing 1 times of EIA detection. Methods To collect data of HIV 1/2 screening positive results and confirmatory test for voluntary blood donors from 2009 to 2014 in Jiaxing area, and to compare the relationship of sereeing test results with that of the confirmatory test, and then to analyze the relevance between S/CO values of screening test and confirmatory test. Results Screening positive rates of domestic and imported reagents, which were 9. 58/10 000 and 12. 43/10 000,respectively;and the confirmatory coincidence rates were 11.84% and 9. 12%, respectively.There was no significant difference （X2= 1.11, P〉0. 05）. The double-reagent joint detection positive rate was 1.37/10 000, and its positive predictive value was 82. 86%.Single-reagent test result compared with that of double-reagent test,which had significant differences （X2domestic = 94. 04, P〈0. 05 andX2imported = 124. 86, P〈0. 05）.When the S/CO value was more than 6, domestic and imported reagents positive predictive values were 93.55% （29/31） and 87.50% （28/32）, respectively. Conclusion There is no difference between domestic and imported reagents EIA-HIV1/2.
出处
《中国输血杂志》
北大核心
2017年第7期813-815,共3页
Chinese Journal of Blood Transfusion
