摘要
背景:骨桥蛋白是一种细胞外多功能基质糖蛋白,已被证实参与调控骨关节炎软骨细胞外基质成分的合成分解代谢,但对于骨关节炎软骨细胞本身增殖能力的影响,报道较少。目的:观察骨桥蛋白对人膝骨关节炎软骨细胞增殖能力的影响。方法:所有软骨标本均取自湘雅医院于2012年1至6月因骨关节炎行膝关节置换患者,分离培养骨关节炎软骨细胞,分别予以骨桥蛋白干预、骨桥蛋白sh RNA慢病毒转染、空白对照及Con-shR NA转染等处理,利用MTT及BrdU法检测细胞的增殖能力变化。结果与结论:(1)骨桥蛋白shR NA慢病毒对骨关节炎软骨细胞转染率为80%;(2)与对照组相比,骨桥蛋白干预可明显上调骨关节炎软骨细胞吸光度值(P<0.05),而骨桥蛋白sh RNA慢病毒干扰组吸光度值则显著下降(P<0.05);(3)骨桥蛋白shR NA转染后,骨桥蛋白的表达水平显著下降(P<0.05);(4)结果表明骨桥蛋白可促进骨关节炎软骨细胞增殖,有望成为治疗骨关节炎的新靶点。
BACKGROUND： Osteopontin, a kind of extracellular matrix glycoprotein, has been found to participate in synthesis and catabolism of osteoarthritic chondrocyte extracellular matrix. However, the effect of osteopontin on the proliferation ability of osteoarthrJtic chondrocytes is little reported. OBJECTWE： To investigate the effect of osteopontin on the chondrocyte proliferation in human knee osteoarthritis. METHODS： Cartilage samples were obtained from the patients with knee osteoarthritis undergoing knee arthroplasty at the Xiangya Hospital from January 2012 to June 2012. The chondrocytes were isolatedand cultured, and then divided into four groups： blank control, osteopontin, Con-shRNA and osteopontin-shRNA groups. The cell proliferation was detected by MTT and BrdU assays. RESULTS AND CONCLUSION： After transfection of osteopontin-shRNA [entivirus, the infection rate was up to 80%. Compared with the blank control group, osteopontin group showed a significant increase in the absorbance value of osteoarthritic chondrocytes, while after osteopontin-shRNA lentivirus transfection, the absorbance value was significantly decreased （both P 〈 0.05）. Additionally, after osteopontin-shRNA transfection, the expression level of osteopontin was significantly downregulated （P 〈 0.05）. To conclude, osteopontin can promote the proliferation of osteoarthritic chondrocytes, which is considered as a new treatment target for osteoarthritis.
出处
《中国组织工程研究》
CAS
北大核心
2017年第24期3773-3777,共5页
Chinese Journal of Tissue Engineering Research
基金
深圳市科技创新项目(JCYJ20150403101028191)~~
