摘要
为筛选禽致病性大肠杆菌(APEC)中与phoP/Q二元调控系统相关的耐药基因表达谱情况并对耐药相关基因进行分析,本实验采用基因芯片技术对APEC野生株和△phoP/Q基因缺失突变株进行转录组的差异性比较,结果检测到713个差异表达基因,其中403个基因的表达水平上调,310个基因的表达水平下调。这些基因涉及生物过程、细胞组分、蛋白质分类及调控通路等功能,本研究着重从上述差异基因中筛选出了aphA、parE等耐药相关的功能基因。本研究对phoP/Q二元调控系统与APEC耐药调控的相关性进行了探索与分析,为进一步深入探寻APEC耐药性的调控机制提供了新的切入点与研究靶标。
To select and analyze the antimicrobial genes related to phoP/Q two-component system in avian pathogenic Escherichia coli（APEC）,we employed gene chips to profile changes in gene expression of APEC wild strain and phoP/Q gene deletion mutant strain.The chip analysis showed that fluorescence intensity of 713 hybridization dots were obviously screened out,of which 403 gene expression were up-regulated and 310 down-regulated.These genes were involved in many biological processes,such as cellular component,protein classification and regulatory pathway.Some antibiotic-resistance genes were screened out,such as aphA,parE and so on.In this study,we explored and analyzed the correlation between phoP/Q two-component system and antibiotic-resistance regulation of APEC,which could provide new entry point and research target for the exploration of the regulatory mechanism in antibiotic-resistance of APEC.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2017年第8期672-674,共3页
Chinese Journal of Preventive Veterinary Medicine
基金
国家自然科学基金项目(31372402)
2016~2018年安徽高校科研创新平台团队建设项目:动物病原生物学(皖教秘科[2015]49号)
