摘要
目的探讨白杨素(Ch R)对膀胱癌T24细胞裸鼠移植瘤生长的影响。方法建立人膀胱癌T24裸鼠皮下异体移植模型(n=40)。接种4周全部成瘤后,将动物随机分为5组:对照组(n=8):腹腔内注射生理盐水,隔日给药1次;丝裂霉素(MMC)组(n=8):腹腔内注射MMC,4 mg/kg,隔日给药1次;低剂量Ch R组(L-Ch R,n=8):腹腔内注射Ch R,20 mg/kg,隔日给药1次;中剂量Ch R组(M-Ch R,n=8):腹腔内注射Ch R,40 mg/kg,隔日给药1次;高剂量Ch R组(H-Ch R,n=8):腹腔内注射Ch R,80 mg/kg,隔日给药1次。连续治疗20 d,裸鼠每周称重2次,测量肿瘤2次,治疗结束处死动物,取出肿瘤称重计算抑瘤率。结果 Ch R(20 mg/kg、40 mg/kg、80 mg/kg)对人膀胱癌T24细胞皮下移植瘤瘤重抑制率分别是40.5%、62.8%和73.4%,与对照组比较,移植瘤重量显著降低(P<0.05);不同剂量Ch R组裸鼠的白细胞计数、血清谷丙谷草转氨酶、尿素氮及肌酐与生理盐水模型对照组相比无显著性差异(P>0.05)。结论 Ch R能显著抑制人膀胱癌裸鼠移植瘤生长,呈剂量和时间依赖性,是一种毒副作用较小的治疗膀胱癌新候选药物。
Objective To investigate the inhibition effect of chrysin（Ch R） on the growth of xenotransplanted tumor with human bladder cancer cell line in vivo. Methods Xenotransplanted model of a human bladder cancer cell T24 in the dorsal skin of nude mice was established. Four weeks later, 40 mice were randomly divided into 5 groups.Control group（n=8）： 0.9% NS injected intraperitoneally on alternate days; MMC group（n=8）： MMC was injected intraperitoneally at dose of 4 mg/kg on alternate days; L-Ch R group（n=8）： Ch R was injected intraperitoneally at dose of 20 mg/kg on alternate days; M-Ch R group（n=8）： Ch R was injected intraperitoneally at dose of 40 mg/kg on alternate days; H-Ch R group（n=8）： Ch R was injected intraperitoneally at dose of 80 mg/kg on alternate days. Twenty days after treatment, tumor weight, size and tumor growth inhibition rate were evaluated respectively. Results After treatment with Ch R in nude mice model, the tumor growth inhibition rate of Ch R in 20 mg/kg, 40 mg/kg, 80 mg/kg concentration was 40.5%, 62.8% and 73.4% respectively, which was significantly different compared with control group treated with NS（P〈0.05）. The changes of WBC, serum ALT, AST, BUN and creatine levels in nude mice of each Ch R group were not significantly different compared with control group treated with（P〈0.05）. Conclusion Ch R can inhibit the tumor growth of human bladder cancer line T24 cells in nude mice and show a dose-and time-dependence. It may be a new candidate for the treatment of bladder cancer with low toxicity.
作者
李卿
何娅娣
罗子寰
陈晓波
高新
李辽源
Li Qing He Yadi Luo Zihuan Chen Xiaobo Gao Xin Li Liaoyuan(Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China Center of Physical Examination Department of Urology, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.)
出处
《中华腔镜泌尿外科杂志（电子版）》
2017年第4期53-56,共4页
Chinese Journal of Endourology(Electronic Edition)
基金
国家自然科学基金(81502206)
关键词
膀胱肿瘤
白杨素
裸鼠
移植瘤
Bladder cancer
Chrysin
Nude mice
Xenotransplanted tumor
