摘要
目的:对神香草40%乙醇洗脱物进行成分分析,并考察其对哮喘小鼠炎症的改善作用。方法:收集聚酰胺树脂柱40%乙醇洗脱物,采用高效液相色谱-高分辨率质谱技术对神香草40%乙醇洗脱物成分进行分析。将72只小鼠随机分为空白组(生理盐水)、模型组(生理盐水)、地塞米松组(阳性对照,1.6 mg/kg)和神香草40%乙醇洗脱物高、中、低剂量组(200、100、50 mg/kg),每组12只。除正常组外,其余各组小鼠均于实验第0、14天ip 0.2 m L卵白蛋白(OVA)致敏;于实验第25~31天ig给药,每天1次;给药结束后,用2 mg/mL OVA连续滴鼻激发7 d。末次滴鼻激发24 h后,检测各组小鼠肺泡灌洗液(BALF)中肿瘤坏死因子α(TNF-α)、白细胞介素4(IL-4)和干扰素(IFN-γ)水平;观察肺组织病理变化。结果:共鉴定出11种化合物,相对百分含量为40.89%,主要成分有迷迭香酸、木犀草素7-O-α-L-吡喃鼠李糖(1→6)-β-D-吡喃葡萄糖苷、金丝桃苷等。与空白组比较,模型组小鼠BALF中TNF-α、IL-4水平升高,IFN-γ水平降低,IL-4/IFN-γ比值增大(P<0.01);肺组织破坏严重,血管周围有炎症细胞浸润。与模型组比较,地塞米松组和神香草40%乙醇洗脱物高、中剂量组小鼠BALF中TNF-α、IL-4水平降低,IFN-γ水平升高,IL-4/IFN-γ比值减小(P<0.01);肺组织病理变化改善。结论:本研究建立的分析方法能够有效地分析神香草40%乙醇洗脱物中的化学成分;神香草40%乙醇洗脱物可调节哮喘小鼠肺组织炎症因子的释放,减轻哮喘小鼠肺组织的炎症病变。
OBJECTIVE： To conduct component analysis for the 40% ethanol eluate of Hyssopus officinalis, and investigate its improvement effect on inflammation in asthmatic mice. METHODS： The 40% ethanol eluate of polyamide resin column was col- lected, and HPLC-high resolution mass spectrometry was used for the component analysis of 40% ethanol eluate of H. officinalis. Totally 72 mice were randomly divided into blank group （normal saline）, model group （normal saline）, dexamethasone group （positive control, 1.6 mg/kg） and 40% ethanol eluate of H, officinalis high-dose, medium-dose, low-dose groups （200, 100, 50 mg/kg） , 12 in each group. Except for normal group, mice in other groups were intraperitoneally injected 0.2 mL ovalbumin （OVA） for sensitization in 0, 14 d and intragastrically administrated in 25-31 d, once a day. After administration, 2 mg/mL OVA was dropped in nose for 7 d. After 24 h of last dropping in nose, tumor necrosis factor α （TNF-α）, interleukin-4 （IL-4）, interfer- on-γ （IFN-γ） levels in bronchoalveolar lavage fluid （BALF） were detected; pathological changes in lung tissue were observed. RE- SULTS： Totally 11 compounds were identified, the relative percentage content of 40.89%. The main components were rosmarinic acid, luteolin 7-O-β-D-rhamnoserhamnose （1→6）-α-D-pyran glucoside, hyperoside, etc. Compared with blank group, TNF-α, IL-4 levels in BALF in model group were increased, IFN-γlevel was declined, and IL-4/IFN-γ ratio was enlarged （P〈0.01） ; lung tissue was seriously damaged, there was infiltration of inflammatory cells around the blood vessels. Compared with model group, TNF-α, IL-4 levels in BALF in dexamethasone group, 40% ethanol eluate of H. officinalis high-dose, medium-dose groups were declined, IFN-γ level was increased, and IL-4/IFN-γ ratio was reduced （P〈0.01） ; pathological changes in lung tissue were improved. CONCLUSIONS： The established analysis method can effectively analysis the chemical components of 40% etha
出处
《中国药房》
CAS
北大核心
2017年第25期3532-3535,共4页
China Pharmacy
基金
国家自然科学基金资助项目(No.81560644)
新疆维吾尔自治区中医民族医药科技人才培养项目(No.2016-03-03)
关键词
维药
神香草
40%乙醇洗脱物
支气管哮喘
成分分析
炎症因子
小鼠
Wei medicine
Hyssopus officinalis
40% ethanol eluate
Bronchial asthma
Component analysis
Inflammatory factors
Mice
