摘要
目的探讨髓样分化分子88（myeloid differentiation factor88，MyI）38）在小鼠异位气管移植中的作用。方法采用BALB／c小鼠到C57BL／6小鼠的异位气管移植作为同种异体排斥反应模型，以MyD88抑制剂作为干预措施，并将同系小鼠异位气管移植作为阴性对照组，将BALB／c小鼠到C57BL／6小鼠的异位气管移植模型作为阳性对照组。分别在第7天和14天收集移植气管组织标本进行病理学检查，观察病理学变化，并进行管腔闭塞率和上皮脱失率的半定量分析，确定干预效果。结果（1）移植气管的病理结果显示：在术后第7天，与阳性对照组相比，给药组的气管结构较清晰，上皮细胞脱落显著降低；在术后第14天，给药组的气管管腔形态较阳性对照组明显改善，且与阴性对照组差别不大，上皮细胞也较少脱落。（2）半定量分析结果显示：在术后第7天，与阳性对照组相比，给药组的管腔闭塞率明显降低，P〈0．01，但是上皮细胞的脱失并没有得到改善，P〉O．05；在术后第14天，与阳性对照组比较，给药组的管腔闭塞率降低，P〈O．05，与术后第7天不同的是，上皮脱失率也明显降低，P〈O．叭。结论在小鼠异位气管移植模型中，抑制MyD88能明显减轻移植气管的病理改变，其管腔闭塞率和上皮细胞脱失率都有所下降。
Objective To explore the role of MyD88 in heterotopic tracheal transplantation in mice and its relationship with histopathological changes. Methods The mouse model of heterotopictracheal transplantation was use＆ The mice were divided into three groups： （1） tracheal isograft of C57BL/6 to C57BL/6 mice; （2） tracheal allograft of BALB/c to C57BL/6 mice; （3） tracheal graft of BALB/c to C57BL/6 mice with MyD88 inhibitor treatment. The tracheal grafts were collected at indicated time points. Histological sections were stained with hematoxylin and eosin. The pathological changes were observed and their semi-quantitative measurement was done with Image J software. Results （1） Pathological results showed that the structure of the trachea with MyD88 inhibitor treatment was clear and the loss of epithelial cells was significantly reduced as compared with the positive control group at the time of 7 and 14 days. （2） The results of semi-quantitative measurement showed that luminal occlusion rate of MyD88 inhibitor treatment group was significantly reduced as compared with the positive control group （P〈0. 01）. However, the loss of epithelial cells was not improved 7 days after transplantation. Both of lumen occlusion rate （P〈0. 05） and epithelial cells loss （P〈0. 01） in MyD88 inhibitor treatment group were significantly reduced. Conclusion Inhibition of MyD88 molecule could significantly alleviate pathological changes of the transplanted trachea. Both of luminal occlusion rate and loss oI epithelial cells were signilicantly amehoratecl.
出处
《中华器官移植杂志》
CAS
CSCD
2017年第7期385-389,共5页
Chinese Journal of Organ Transplantation
基金
国家自然科学基金面上项目（81471588）
