摘要
目的研究HBx对肝卵圆细胞发生恶性转化并上皮-间质转化(EMT)的影响。方法以稳定表达HBx的转基因卵圆细胞株pEGFP-HBx-LE/6为实验组,pEGFP-LE/6卵圆细胞为对照组。使用Western blotting、Transwell细胞侵袭实验、划痕实验、CCK-8等检测pEGFP-HBx-LE/6在细胞周期、分化标志物以及增殖转移能力方面的变化。结果(1)实验组细胞体积增大、具有明显异形性,CK-19、CK-7表达明显下降,AFP、HNF-4α表达升高;(2)实验组细胞的增殖能力明显升高,克隆形成能力、侵袭能力、划痕恢复能力明显增强;(3)与对照组比较,实验组E-cadherin表达水平明显下降,而N-cadherin、α-SMA、Vimentin表达水平明显上升。结论 HBx基因可诱导肝卵圆细胞向恶性转化,并促进其转移。
Objective To investigate the effect of HBx gene on the tumorigenesis and metastatic ability of hepatic oval cells.Methods HBx gene or vector gene was transfected into hepatic oval cell line LE/6,serving as experimental group and control group,respectively.Western blotting,Transwell assay,and CCK-8assay were applied to explore the effect of HBx gene on the tumorigenesis and metastatic ability of oval cells.Results（1）The volume of cells in the experiment group was increased and the atypia was obvious.The expression of CK-19 and CK-7was significantly decreased,and that of AFP and HNF-4αincreased;（2）The proliferation of pEGFP-HBx-LE/6cells was significantly increased.The colony forming ability,invasion ability and scratch recovery ability were enhanced obviously;（3）As compared with the control group,the expression level of E-cadherin was significantly decreased,and that of N-cadherin,α-SMA and Vimentin increased significantly in the experimental group.Conclusion HBx gene is confirmed to promote the metastatic ability and tumorigenesis of hepatic oval cells.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2017年第4期410-413,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金青年基金资助项目(No.81402410
No.81602626)
