摘要
本研究采用RP-HPLC法测定赣南产金沙藤中异槲皮苷和紫云英苷的含量,色谱柱为Agilent C_(18)柱,规格为(150 mm×4.6 mm,5μm);流动相为甲醇(B)-水(A),流速1.0 m L/min,柱温30℃,检测波长为258 nm。结果显示14 min内异槲皮苷和紫云英苷分离度良好,异槲皮苷和紫云英苷的线性范围分别是0.349~4.188μg(r=0.999 8,n=5)、0.603~4.824μg(r=0.999 5,n=5),平均加样回收率分别为98.57%(RSD=1.45%)、96.96%(RSD=1.62%)。表明RP-HPLC法重复性好,比较稳定,操作方便,适用于测定金沙藤中异槲皮苷和紫云英苷的含量。
This study measured isoquercitrin and astragalin in Lygodii Herba produced in Gannan by RP-HPLC. The column was Agilent C18 （150 mm×4.6 mm, 5μm）, the mobile phase was methanol （B）-water （A） with the flow rate of 1.0 mL/min and the column temperature of 30℃, and the detection wavelength was set at 258 nm. The result showed the good separation of Isoquercetin and Astragalin was achieved within 14 minutes. The linear range ofisoquercitrin and astragalin were 0.349-4.188 μg （r=0.999 8, n=5） and 0.603-4.824 μg （r=0.999 5, n=5）, respectively. The average recoveries of isoquercitrin and astragalin were 98.57% （RSD=1.45%） and 96.96% （RSD=1.62%）, respectively. It implied that RP-HPLC was of high repetitiveness, good stability and simple operation, thus it could be applied to determine the content of isoquercetin and astragalin in Lygodii Herba produced in Gannan.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第8期3133-3137,共5页
Genomics and Applied Biology
基金
江西省科技厅青年科学基金项目(20161BAB215220)
赣州市社会科学研究课题(16088)
赣南医学院校级科研课题(YB201609)共同资助
