摘要
目的:研究毛蕊异黄酮对D-半乳糖致衰老小鼠学习记忆能力的改善作用及作用机制。方法:小鼠皮下注射D-半乳糖150 mg·kg^(-1)·d^(-1),连续6周,造模后的小鼠分为模型组、奥拉西坦组(300 mg·kg^(-1)·d^(-1))、和毛蕊异黄酮高、中、低剂量组(20、12、7 mg·kg^(-1)·d^(-1))。模型组和正常对照组小鼠给予等剂量的生理盐水。处理7 d后,利用跳台和Y迷宫实验检测空间记忆和被动学习能力,测定肝、脾、胸腺和脑器官指数,检测血清总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)含量和脑组织中乙酰胆碱酯酶(Ach E)活力。结果表明,与模型组相比,毛蕊异黄酮处理组小鼠在Y迷宫中正确反应次数和主动回避率明显增加(p<0.05),在跳台实验中逃避期均明显缩短,错误次数明显减少(p<0.05);毛蕊异黄酮处理组小鼠肝、脾、胸腺和脑器官指数与正常对照组没有明显差异;抗氧化酶(T-SOD、GSH-Px)活力显著升高(p<0.05),MDA含量明显降低,脑组织中Ach E活性降低(p<0.05)。结论:毛蕊异黄酮对D-半乳糖致衰老小鼠学习记忆能力有改善作用,毛蕊异黄酮可能是通过提高内源抗氧化酶(T-SOD、GSH-Px)活力,降低脂质过氧化损伤,保护脑、脾、胸腺等器官功能和降低脑组织Ach E活性来提高D-半乳糖致衰老小鼠的学习记忆能力。
Objective： The effects of calycosin on learning-memory behavior in aging mice induced by D- galactose were investigated in this study. Methods： The aging mice model was induced by subcutaneous injection of D- galaetose （150 mg·kg^-1·d^-1） were established after 6 weeks. The D-galactose-induced aging mice were divided into model group, oxiracetam group （ 300 mg- kg^-1·d^-1） , and calycosin high, medium and low dose treated- groups （ 20,12,7 mg·kg ^- 1·d^ -1）. Mice in the model group and normal control group were given normal saline. After intraperitoneal injection 7 d, the passive learning ability and memory capacity of mouse were investigated by the platform and the Y maze. Then organ index and antioxidant activity were detected, such as liver, spleen, thymus, brain and serum superoxide dismutase （ T- SOD ） , glutathione peroxidase（GSH-Px）, malondialdehyde （MDA）and brain aeetylcholinesterase （AchE）activity. Results： Compared with the model group, in the Y maze, the number of correct reaction and active avoidance rate of calycosin- treated mice were significantly increased（p 〈 0.05 ） , and the escape period was significantly shortened and the number of errors in the step-down test was significantly reduced （p 〈 0.05）.Compared with the model group, liver, spleen, thymus and brain organ index of calycosin- treated mice were significantly different （p 〈 0.05 ）. In the calycosin groups, antioxidant enzyme （ T- SOD, GSH- Px ） activity was significantly elevated （p 〈 0.05 ）, MDA content was significantly reduced （p 〈 0.05 ）, and brain tissue AchE activity was decreased （ p 〈 0.05 ） . Conclusion ： Calycosin can improve the learning - memory function of aging mice induced by D-galactose. The mechanism may be to increase the endogenous antioxidant enzyme activity, reduce lipid peroxidation damage, protect organ function such as brain, spleen and thymus and reduce brain tissue AchE activity.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第17期292-295,共4页
Science and Technology of Food Industry
基金
河北省教育厅项目(Z2011247)
