摘要
探讨了ω-3PUFAs对大鼠心肌细胞代谢及血清T-SOD、MDA、血乳酸、LDH、IL-4、IL-6、INF-γ干预作用。将SD大鼠分为安静对照(Rc)组、运动对照(Mc)组、低剂量ω-3PUFAs灌胃(G1)组、中剂量ω-3PUFAs灌胃(G2)组和高剂量ω-PUFAs灌胃(G3)组。对SD大鼠进行8周递增负荷跑台运动复合ω-3PUFAs干预实验。结果表明:实验组大鼠心肌细胞高能磷酸化合物含量高于Mc组;实验组血清T-SOD活力呈现上升趋势;实验组MDA含量呈现下降趋势,G1组与Mc组比较具有显著差异(P<0.05),G2、G3组与Mc组比较具有极显著差异(P<0.01);实验组血乳酸水平呈现下降趋势,G1、G2组与Mc组比较具有显著差异(P<0.05),G3组与Mc组比较具有极显著差异(P<0.01);实验组LDH含量呈现上升现象,且LDH含量与ω-3PUFAs剂量呈正相关;实验组大鼠血清IL-4、IL-6、INF-γ水平低于Mc组。不同剂量ω-3PUFAs对大鼠心肌细胞代谢、抗氧化能力及血清IL-4、IL-6、INF-γ具有干预作用。
In order to investigate the effects of ω- 3PUFAs on the myocardial cell metabolism and the changes of serum T - SOD, MDA, blood lactate, LDH, IL - 4, IL - 6 and INF -γ in rats, the SD rats were divided into sedentary control （Re） group, exercise control （Me） group, low dose ω-3PUFAs ga- vage （ G1 ） group, medium dose ω- 3PUFAs gavage （G2） group and high dose ω- 3PUFAs gavage （ G3 ） group, and a compound ω-3PUFAs intervention experiment by treadmill exercise for eight weeks was carried out. The results showed that the contents of high-energy phosphate compounds in experimental group were higher than those in Mc group. Serum T- SOD activity in experimental group showed an upward trend, while MDA content showed a downward trend, and G1 group had a significant difference with Mc group groups had highly signifi （ P 〈 0.05 ）, G2 and G3 cant differences with Mc group （P 〈 0.01 ）. Blood lactate levels in experi-mental group showed a downward trend. G1 （ P 〈 0.05 ）, and G3 group had highly signifi and G2 groups had significant differences with Mc group cant differences with Mc group（ P 〈0.01 ）. LDH content in experimental group showed an upward trend, and it was positively correlated with to -3 PUFAs dose. Serum IL -4, IL- 6, INF -γ levels in experimental group rats were lower than those in Mc group. Dif- ferent doses of ω - 3 PUFAs had intervention on the myocardial cell metabolism, antioxidant capacity and serum IL - 4, IL - 6, INF - γ in rats.
出处
《中国油脂》
CAS
CSCD
北大核心
2017年第8期63-66,共4页
China Oils and Fats
基金
国家自然科学基金(31660736)
2016年新疆维吾尔自治区研究生科研创新项目(XJGRI2016106)
