摘要
目的探讨缬沙坦对糖尿病大鼠肾脏klotho蛋白(KL)和氧化应激的影响。方法糖尿病大鼠随机分为糖尿病(DM)组和缬沙坦干预(VA)组,另设对照(NC)组,每组20只。VA组予缬沙坦55mg/(kg·d)灌胃,NC组及DM组予等量生理盐水灌胃,于4周及12周测定大鼠肾功能、血清KL、8-羟基脱氧鸟苷(8-OHdG)及超氧化物歧化酶(SOD)活性,免疫组织化学测定肾脏KL的表达水平。结果与NC组比较,DM组4周和12周时KL含量降低[(4.39±0.35)vs(3.73±0.69)ng/ml,(4.16±0.28)vs(3.30±0.37)ng/ml],SOD活性降低[(1.87±0.84)vs(1.69±0.29)U/ml,(1.79±0.48)vs(1.50±0.26)U/ml],8-OHdG增加[(2.74±0.11)vs(3.32±0.91)ng/ml,(2.86±0.28)vs(3.21±0.25)ng/ml](P<0.05);与DM组比较,VA组上述指标亦改善,差异有统计学意义(P<0.05)。结论糖尿病大鼠KL的表达降低,缬沙坦可提高糖尿病大鼠肾脏KL的表达,抑制氧化应激反应。
Objective To investigate the effects of Valsartan on klotho protein （KL） and oxidative stress in diabetic rat. Methods Diabetic rat models were randomly divided into diabetes group （DM group,n=20） and Valsartan intervention group （VA group,n=20）. Healthy rats were selected as normal control group （NC group, n= 20）. VA group were treated by Valsartan 55 mg/（kg·d） continuous lavage. DM group and NC group were given amount of saline lavage daily. Renal function, serum KL, 8- OHdG,and SOD were detected after follow up for 4 and 12 weeks. KL in kidney were detected by immunohistochemical method. Results KL at 4 weeks and 12 weeks were significantly reduced in DM group than in NC group [（3.73＋0. 69） vs （4. 39±0.35） ng/ml, （3.30±0.37） vs （4.16±0.28） ng/ml,P d0. 05]. SOD at 4 weeks and 12 weeks were significantly reduced in DM group than in NC group [（1.69±0. 29） vs （1.87±. 84） U/ml,（1. 50±0. 26） vs （1. 79±0.48） U/ml,P%0.05]. 8-OHdG at 4 weeks and 12 weeks significantly increased in DM group than in NC group [（3.32±0. 91） vs （2. 74±0. 11） ng/ml, （3.21±0. 25） vs （2.86±0.28） ng/ml,P〈0.05]. All these parameters were remarkably improved in VA group （P《0. 05）. Conclusion KL decreases in diabetic rats. Valsartan can increase KL expression,and inhibit the oxidative stress in diabetic rats.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2017年第8期733-736,共4页
Chinese Journal of Diabetes
基金
黑龙江省教育厅科学技术研究项目(12521196)
