摘要
目的 探讨不同浓度高渗钠盐溶液（HS）对严重烫伤大鼠早期肝损伤的影响.方法 按随机数字表法将104只雌性SD大鼠分为假伤组（n=8）、乳酸钠林格液（LR）组（n=24）及600、800、1000 mmol/L HS组（HS600组、HS800组、HS1000组,n=24）5组.LR组及各HS组大鼠在30%总体表面积（TBSA）Ⅲ度烫伤后分别经尾静脉输注LR及相应浓度的HS进行液体复苏,并于补液后2、8、24 h取腹主动脉血后处死大鼠取肝脏组织;假伤组大鼠模拟烫伤后直接处死取标本.采用全自动生化分析仪检测血清丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）水平;用紫外分光光度计检测肝组织丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性;用蛋白质免疫印迹试验（Western Blot）检测肝组织p38丝裂素活化蛋白激酶（p38MAPK）表达.结果 与假伤组比较,LR组和3个HS组各时间点ALT、AST、MDA及p38MAPK表达均明显增加,SOD活性明显降低.与LR组比较,不同浓度HS组ALT、AST、MDA、p38MAPK表达均有不同程度降低,SOD活性有不同程度升高,其中HS600组改变最为显著〔ALT（U/L）：伤后8 h为147±52比227±60,伤后24 h为138±47比191±41;AST（U/L）：伤后2 h为288±79比548±237,伤后8 h为567±167比841±338,伤后24 h为515±180比712±159;MDA（nmol/mg）：伤后2 h为0.287±0.036比0.395±0.041,伤后8 h为0.298±0.030比0.392±0.018,伤后24 h为0.278±0.033比0.422±0.036;SOD（U/mg）：伤后2 h为230±16比159±30,伤后8 h为251±14比194±15,伤后24 h为296±8比243±11;磷酸化p38MAPK（p-p38MAPK）/p38MAPK（A值）：伤后2 h为0.778±0.040比1.065±0.066,伤后8 h为0.791±0.046比0.967±0.041,伤后24 h为0.733±0.027比1.020±0.043;均P〈0.05〕.不同HS浓度组间比较显示,HS600组ALT、AST于伤后2 h显著低于HS1000组,伤后8 h显著低于HS800组;伤后各时间点MDA含量、p38MAPK表达显著低于HS800组和HS1000组,SOD活性显著高于HS800组和HS1000组;而HS800组与HS1000组伤后各时间
Objective To investigate the effects of three different concentrations of hypertonic sodium salt （HS） resuscitation on liver injury of rats at the early stage of severe burned.Methods 104 female Sprage-Dawley （SD） rats were randomly divided into five groups： sham group （n = 8）, lactated Ringer solution （LR） group （n = 24）, 600, 800, 1000 mmol/L HS groups （HS600, HS800, and HS1000 groups,n = 24）. Rats in LR group and HS groups were subjected to full-thickness scald with 30% total body surface area （TBSA）, and then given liquid resuscitation treatment with LR and the corresponding HS. These rats were sacrificed at 2, 8 and 24 hours post injury to collect blood and liver tissue. Rats in sham group were given simulation of burns without resuscitation, which were immediately sacrificed and the specimens were harvested. The levels of serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were detected by automatic biochemical analyzer. The levels of liver tissue malondialdehyde （MDA） and superoxide dismutase （SOD） were detected by ultraviolet spectrophotometry. The expression of liver tissue p38 mitogen-actirated protein kinase （p38MARK） was detected by Western Blot.Results Compared with sham group, the levels of ALT, AST, MDA and p38MAPK were increased, and the activities of SOD were decreased in LR group and different degrees in HS groups at each time point after injury. Compared with LR group, the levels of ALT, AST, MDA and p38MAPK were decreased and the activities of SOD were increased in different degrees with HS groups, among which HS600 group changed most significantly [ALT （U/L）： 147±52 vs. 227±60 at 8 hours, 138±47 vs. 191±41 at 24 hours; AST （U/L）：288±79 vs. 548±237 at 2 hours, 567±167 vs. 841±338 at 8 hours, 515±180 vs. 712±159 at 24 hours; MDA （nmol/mg）： 0.287±0.036 vs. 0.395±0.041 at 2 hours, 0.298±0.030 vs. 0.392±0.018 at 8 hours, 0.278±0.033 vs. 0.422±0.036 at 24 hours; SOD （U/mg）： 230±16 vs. 15
出处
《中华危重病急救医学》
CAS
CSCD
北大核心
2017年第8期726-730,共5页
Chinese Critical Care Medicine
基金
国家自然科学基金（81272092）
关键词
烧伤
烫伤
高渗盐
丙二醛
超氧化物歧化酶
信号转导通路
Burns
Scald
Hypertonic saline
Malondialdehyde
Superoxide dismutase
Signal transduction pathway
