摘要
为探究猪传染性胃肠炎病毒(TGEV)LY86株与其他不同来源TGEV毒株之间在分子生物学上的差异及其在猪睾丸(ST)细胞上的增殖特性,分别进行了TGEV LY86株N基因的序列分析,以及病毒株以不同感染复数(MOI)感染ST细胞的毒价测定。结果显示:TGEV LY86株与其他17株TGEV参考毒株核苷酸序列同源性在97.2%~99.8%,特别是与M6、M60、TS、H、H16及JS2012毒株之间均存在很高的同源性;感染复数直接影响TGEV LY86株在ST细胞上的增殖效率,过高或过低的感染复数均可导致较低的TGEV增殖效价;当以MOI=0.001接毒时,感染后48 h所收获的病毒毒价最高,达10^(6.20±0.23)TCID_(50)/0.1 mL。本研究对分析TGEV的遗传变异规律与感染特征、优化疫苗生产工艺等具有重要的参考意义。
This study aimed to investigate the differences in molecular biology between porcine transmissible gastroenteritis virus（ TGEV）LY86 strain and other TGEV strains and its proliferation in swine testicular（ ST） cells. The N gene of TGEV YL86 strain was amplified and sequenced,and ST cells were inoculated with TGEV YL86 strain at different multiplicity of infection（ MOI） and virus titers were measured at different time points post infection. The results showed that the N gene of TGEV YL86 strain shared 97. 2% to 99. 8% homology with TGEV reference strains,and there was a high homology between TGEV LY86 strain and M6,M60,TS,H,H16 or JS2012 strains. MOI had an important effect on proliferation of TGEV YL86 strain in ST cells. The virus titer reached peak of 10^（6. 20±0. 23）TCID_（50）/0. 1 mL at 48 hours post infection with an MOI of 0. 001. These results have great significance in analyzing the genetic variation and infectious characteristics of TGEV and optimizing vaccine production process.
出处
《畜牧与兽医》
北大核心
2017年第8期102-106,共5页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31672515)
关键词
猪传染性胃肠炎病毒
猪睾丸细胞
N基因
序列分析
增殖特性
transmissible gastroenteritis virus of swine
ST cells
N gene
sequence analysis
proliferation characteristics
