摘要
目的:分析不同剂量的骨蚀宁胶囊对激素性股骨头坏死(INFH)动物模型蛋白-2(BMP-2)表达的影响,进一步探讨骨蚀宁胶囊治疗INFH的作用机理。方法:选取成年健康新西兰大白兔56只,随机选取6只为正常对照组,剩余50只用于骨坏死造模;造模成功后观察2周,将50只模型动物随机分为5组:骨蚀宁胶囊低剂量组、骨蚀宁胶囊中剂量组、骨蚀宁胶囊高剂量组、空白对照组和阳性对照组,每组10只;继续培养8周,比较6组动物BMP-2表达水平以及血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)等指标。结果:TXB2水平比较为空白对照组>低剂量组>高剂量组>阳性对照组>中剂量组>正常对照组;6-keto-PGF1α水平比较为正常对照组>中剂量组、阳性对照组>高剂量组>低剂量组>空白对照组;BMP-2表达情况为正常对照组>中剂量组>高剂量组>低剂量组、阳性对照组>空白对照组,差异比较有统计学意义(均P<0.05)。结论:骨蚀宁胶囊可以显著改善兔股骨头局部组织瘀血状态,提高BMP-2表达水平,且中剂量组(1.41g/kg·d)用药效果比其他剂量组更佳。
Objective： To investigate the effect of different doses of Gushining capsule on the expression of protein-2（BMP-2） in steroid-induced femoral head necrosis （INFH）, and to further explore the mechanism of the treatment of INFH. Methods： 56 adult New Zealand white rabbits were selected and randomly selected 6 as the normal control group,the remaining 50 for bone necrosis modeling; 2 weeks after modeling success, 50 model animals were randomly divided into 5 groups： low-dose group, Middle dose group, high dose group of Gushining capsule, the control group and positive control group, 10 rats in each group. The levels of BMP-2 and the levels of thromboxane B2 （TXB2） and 6-ketoprostaglandin F1α （6-keto-PGF1α） were compared after 8weeks. Results： TXB2 expression： blank control group〉low dose group〉 high dose group〉 positive control group〉 middle dose group〉 normal control group; 6-keto-PGF1 α expression： normal control group〉 middle dose group, positive control group〉 high dose group〉 low dose group〉 blank control group; BMP-2 expression： normal control group〉 middle dose group〉 high dose group〉 low dose group, positive control group〉 blank control group. The difference was statistically significant （ P〈0.05 ）. Conclusion： Gushining capsule can significantly improve the blood stasis status of the femoral head in rabbits and improve the expression of BMP-2, and the effect of the middle dose group （1.41g / kg·d） is better than that of other dosage groups
作者
江树连
朱磊
李文华
周正新
JIANG Shulian ZHU Lei LI Wenhua ZHOU Zhengxin(The First Affiliated Hospital of Anhui University of Chinese Medicine Anhui Hefei 2300631, China)
出处
《中医药临床杂志》
2017年第7期1042-1044,共3页
Clinical Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81373664)
关键词
破瘀通络法
激素性股骨头坏死
BMP-2
Blood Stasis dredging method
Steroid induced osteonecrosis of the femoral head
BMP-2
