摘要
目的以明胶为主要原料,京尼平为交联剂,包载磺胺嘧啶银纳米晶体制备载药交联凝胶敷料,并探究其理化性质与释药特性。方法球磨法制备Ag SD纳米晶体,测定粒度及分散系数,透射电镜下观察晶体形貌,并采用超高效液相色谱(UPLC)法测定其6 h内相对累计溶出度;分别制备不同交联度载Ag SD纳米晶体与粗粉凝胶敷料,测定溶出度,考察其溶出行为,扫描电镜下观察微观结构,并于6周后同法重复测定载药凝胶及纳米晶体混悬液各项性质,考察稳定性。结果纳米化后的Ag SD多分散系数为0.211,平均粒径267.8 nm,与粗粉相比6 h溶出度大大提高;载药凝胶释药速率与交联度呈负相关,且纳米晶体凝胶释药速率高于相同交联度粗粉凝胶,电镜观察药物晶体未受交联影响,6周后载药凝胶释药性及微观结构皆稳定,而混悬液晶体粒径增大,溶出速率下降。结论新型载药凝胶敷料溶出良好,性质稳定,可为后续及相关研究提供参考。
Objective To prepare a silver sulfadiazine（Ag SD）-nanocrystal loaded hydrogel with gelatin as the main raw material,genipin as crosslinker,and test its physicochemical property and drug release characteristics. Methods Ag SD-nanocrystal was prepared by ball milling,the particle size and polydispersity index were determined,the solubility was studied by an ultra performance liquid chromatography（UPLC）method and the microstructure was observed with transmission electron microscope（TEM）;drug loaded gelatin hydrogel in different crosslinking degrees were prepared,the dissolution rate was studied in the same way as Ag SDnanocrystal and the possible dissolution mechanism was analyzed by fitting curves;the microstructure was observed by scanning electron microscope（SEM）. After 6 weeks,with the same method,all the above mentioned properties of drug loaded hydrogel and Ag SDnanocrystal suspension were determined repeatedly,and at the same time their stability was tested. Results After nanocrystallization,the polydispersity index of Ag SD was 0.211,the mean particle size was about 267.8 nm,the dissolution rate greatly increased within 6 hours compared with that of Ag SD bulk powder. There was a negative correlation between dissolution rate and crosslinking degree,and the nanoparticle gel′s curve was higher than that of the bulk. In terms of stability,Ag SD-nanocrystal in hydrogel was almost in the original state in SEM,while those in suspention had an increasing particle size and decreasing dissolution rate. Conclusion The stability and dissolution of the new drug loaded hydrogel are good,and its data can be used as reference for relevant study.
作者
邵杰
许玉莲
王丽伟
郭代红
SHAO Jie XU Yu-lian WANG Li-wei GUO Dai-hong(Chinese PLA Medical School, Beijing 100853, China Department of Pharmacy, the First Affiliated Hospital of PLA General Hospital, Beijing 100048, Chlna)
出处
《国际药学研究杂志》
CSCD
北大核心
2017年第6期629-633,共5页
Journal of International Pharmaceutical Research
关键词
明胶
磺胺嘧啶银
敷料
纳米晶体
gelatin
silver sulfadiazine
dressing
nanocrystal
