摘要
目的探讨山楂酸对肺癌细胞95D增殖及凋亡产生的影响。方法将山楂酸作用于人的肺癌细胞系95D,通过四甲基偶氮唑蓝(MTT)法检测山楂酸对细胞增殖的影响;通过流式细胞仪Annexin V/PI双染法检测细胞凋亡;通过荧光显微镜检测细胞核的形态变化;采用Western blot方法检测凋亡相关蛋白的影响。结果随着山楂酸浓度增大,95D细胞的增殖率明显降低,早期及晚期凋亡率逐渐增加,与对照组比较差异有统计学意义;荧光电子显微镜观察山楂酸作用后的细胞,可见染色体边集、核碎裂等典型的凋亡改变,而对照组没有相应的变化;Western blot显示,随着山楂酸浓度的增加,Bcl-2蛋白表达逐渐降低,Bax蛋白表达逐渐升高。结论山楂酸可以抑制肺癌95D细胞增殖,且具有浓度依赖性,山楂酸抑制肺癌95D细胞增殖是通过诱导细胞凋亡实现的。
Objective To research the effect of maslinic acid on the proliferation and apoptosis of lung cancer 95D cells. Methods The effects of maslinic acid on proliferation of 95D ceUs was determined by MTT assay, and the apoptosis was analyzed by Annexin V/PI flow cytometric analysis. The morphology of apoptotic cell was detected by fluorescent microscopy. The effect of apoptosis-related proteins was detected by Western blot. Results Maslinic acid inhibited the proliferation of 95D cells in a dose-dependent manner significantly, and the proportion of Annexin V posi- tive cells in drug-treated cells were increased in a dose-dependent manner, there were significant differences compared with control group （ P 〈 0. 05 ）. The observation of fluorescent microscopy showed that treatment with maslinic acid for 24 h could significantly induce the apoptotic cell death with chromatin condensation and nuclear fragmentation, while no corresponding change was found in control group. Western blot showed that the expression of Bcl-2 protein decreased gradually and the expression of Bax protein increased gradually with the increase of maslinic acid＇s concentration. Con- elusion Maslinic acid could inhibit the proliferation of 95D cell lines in a dose-dependent manner, which is achieved by induction of apoptosis.
出处
《实用药物与临床》
CAS
2017年第7期737-740,共4页
Practical Pharmacy and Clinical Remedies
关键词
山楂酸
肺癌
增殖
凋亡
Maslinic acid
Lung cancer
Proliferation
Apoptosis
