摘要
[目的]研究泽泻汤中成分白术内酯Ⅱ和Ⅲ在大鼠不同肠段的跨膜吸收特征。[方法]采用Agilent Zorbax Plus C18色谱柱,流动相为乙腈-0.1%甲酸水(42∶58),流速:0.3 mL/min。质谱电喷雾离子源,阳离子MRM模式,白术内酯Ⅱ和Ⅲ的定量离子分别为m/z233.2→m/z187,m/z249.15→m/z231。尤斯灌流室法考察泽泻汤在大鼠离体十二指肠、空肠、回肠、结肠的吸收,接收侧样品10 000 r/min离心15 min后用液相色谱-质谱色谱法(LC-MS/MS)分析。[结果]白术内酯Ⅱ在大鼠不同肠黏膜的表观渗透系数范围在6.10~25.22×10^(-6) cm/s之间,白术内酯Ⅲ在1.88~5.41×10^(-6)cm/s之间,两者最佳吸收部位是结肠。[结论]该方法专属性好,准确度高,可用于泽泻汤中白术内酯Ⅱ和Ⅲ的大鼠肠吸收研究。
[Objective] To investigate ratintestinal absorption of Atractylenolide Ⅱ and Atractylenolide Ⅲ in Zexie decoction. [Methods] The column was Agilent Zorbax Plus C18 （4.6 mm × 12.5 mm, 5 μm）. The mobile phase consisted of a mixture acetonitrile-0.1%formic acid aqueous solution （42 ：58）, the flow rate was 0.3 mL/min. Quantification was performed in the positive ionization by MRM mode. The monitored transitions were set at m/z 233.2- m/z187 and m/z 249.15μm/ z231for A tractylenolide Ⅱ and A tractylenolide Ⅲ , respectively. [Results] The apparent permeability coefficients （Pap） of Atractylenolide H was gradually on the increase in duodenum, jejunum, ileum and colon. The same thing happened to the Papp of Atractylenolidein Ⅲ in jejunum, duodenum, ileum and colon. [Conclusion] This method was simple, precise and repeatable, which could be used for intestinal investigation of Zexie decoction.
作者
朱蕴
顾星
张兵
祁东利
刘志东
魏子斌
ZHU Yun GU Xing ZHANG Bing QI Dong- li LIU Zhi- dong WEI Zi- bin(Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China Engineering Research Center of Modern Chinese Medicine Discovery and PreparationTechnique, Ministry of Education, Tianjin 300193, China)
出处
《天津中医药大学学报》
CAS
2017年第4期299-302,共4页
Journal of Tianjin University of Traditional Chinese Medicine
基金
新世纪优秀人才支持计划(NCET-12-1068)
