摘要
目的探讨大鼠心肾联合移植模型手术方法。方法心肾联合移植组雄性近交系Lewis大鼠做为肾移植的供、受者,原位灌注取左肾及心脏,肾动脉重建用套袖吻合法,肾静脉用内撑法间断吻合,左肾原位移植后将供体心脏主动脉和肺动脉分别与受体腹主动脉和下腔静脉端侧吻合,受体右肾立即切除,以Lewis大鼠右肾切除为对照组。结果心肾热缺血时间10 s,供体取肾时间(30.0±3.0)min,供心摘取时间(6.0±1.8)min,冷缺血时间肾脏(40±3.2)min、心脏(70±4.1)min,受体手术时间(95±5.4)min,实施移植手术21例。手术成功率为80.9%,术后30 d肾功能及心率与对照组比较差异无统计学意义。结论建立大鼠心肾联合移植模型稳定可靠,可作为心肾联合移植的理想模型,可应用于多脏器移植免疫的基础研究。
Objective To explore the surgical procedure for combined heart and kidney transplantation in rats.Methods Inbred Lewis rats were used as donors and recipients.The left kidney and the heart were removed from the donors,the donor kidney was therefore transplanted to the left side.Revascularization of renal artery was constructed end-in-end by modified sleeve anastomosis.Renal veins were anastomosed using stenting technique.Ureters were anastomosed using end-to-end interrupted suture technique.The aortic and pulmonary arteries were anastomosed to the recipient abdominal aorta and inferior vena cava Lewis rats receiving right nephrectomy were assigned to a control group.Results The time of warm ischemia was 10s,and the donor nephrectomy time was（30±3.0）min;the removal of donor heart was（6±1.8）min,the renal cold ischemia time was（40±3.2）min and heart was（70±4.1）min,the meantime for recipients operation was（95±5.4）min,and the graft survival rate was 80.9%.Renal function and heart rate did not differ significantly between the two groups 30 days after the procedure.Conclusions The new model for simultaneous heart and kidney transplantation in rats is feasible and reliable.This technique can be applied to basic research on multiple organ transplantation immunity.
出处
《实用医学杂志》
CAS
北大核心
2017年第16期2639-2642,共4页
The Journal of Practical Medicine
基金
国家自然科学基金资助(编号:30701074
81373523)
关键词
肾移植
心脏移植
大鼠
模型
Kidney transplantation
Heart transplantation
Rat
Model
