摘要
旨在探究酰基载体蛋白在植物脂肪酸合成途径中的作用机制,本研究采用传统PCR方法从花生中克隆得到了一个线粒体型基因,命名为AhmtACP3。并采用荧光定量PCR技术分析了基因的表达特性。该基因全长为859 bp,开放阅读框ORF为390 bp,开放阅读框对应的基因组序列为543 bp,由2个外显子和1个内含子组成,该基因编码129个氨基酸,理论分子量为14.5345 k D,理论等电点为5.22,可能定位于线粒体上。系统发育分析表明,花生线粒体型ACP蛋白分成2个分支:AhmtACP1和AhmtACP2有较近的亲缘关系,与AhmtACP3亲缘关系较远。荧光定量PCR结果显示,AhmtACP3基因在花中的表达量明显高于其他组织,其次是子叶和根。AhmtACP3基因在种子发育过程中的表达量呈现下降趋势。
To investigate the mechanism of acyl carrier protein （ACPs） in the pathway of plant fatty acid synthesis, one ACP gene （AhmtACP3） was isolated from peanut by traditional PCR. The expression characteristics of the gene were analyzed by quantitative real-time RT-PCR （qRT-PCR）. The length of AhmtACP3 gene was 859 bp. The length of open reading flame （ORF） was 390 bp, and its genomic sequence was 543 bp with 2 exons and 1 intron. It encoded a protein of 129 deduced amino acid residues. The molecular weight was 14.5345 kD and its isoelectric point was 5.22. The deduced protein was predicted to locate in mitochondria. Phylogenetic analysis indicated that peanut mitochondrial ACPs fell into two clusters. AhmtACP1 was grouped with AhmtACP2, which was distinct from AhmtACP3. qRT-PCR indicated that AhmtACP3 transcripts were more abundant in flowers than in any other tissues tested, followed by cotyledons and roots. The expression levels of AhmtA CP3 were the highest at the initial stage of seed development, but
作者
迟晓元
陈娜
王通
王冕
陈明娜
潘丽娟
郝翠翠
杨珍
梁成伟
禹山林
Chi Xiaoyuan Chen Na Wang Tongl Wang Mian Chen Mingna Pan Lijuan Hao Cuicui Yang Zhen Liang Chengwei Yu Shanlin(Shandong Peanut Research Institute, Qingdao Shandong 266100 Qingdao University of Science ＆ Technology, Biology Department, Qingdao Shandong 266042)
出处
《中国农学通报》
2017年第20期35-42,共8页
Chinese Agricultural Science Bulletin
基金
2014年国家万人计划"青年拔尖人才"
国家花生产业技术体系"良种扩繁与种子生产岗位"(CARS-14)
山东省三院联合基金"花生kennedy途径关键酶基因的分离与功能研究"(ZR2014YL012)
"花生连作根腐病的主效微生物挖掘及其调控因子分析"(ZR2014YL011)
国家自然基金"参与花生脂肪酸合成代谢的关键酶基因的克隆与功能研究"(31000728)
"花生iso-Ara h3基因在种子抗黄曲霉中的功能研究"(31200211)
山东省农业科学院青年英才培养计划"访学研修"
关键词
花生
mtACP3
脂肪酸合成
基因克隆
表达分析
peanut(Arachis hypogaea L.)
mtACP3
fatty acid synthesis
gene cloning
expression analysis
