摘要
目的:建立利用RP-HPLC测定九味羌活丸中阿魏酸和欧前胡素含量的方法。方法:采用C18(4.6 mm×150 mm,5μm)色谱柱,柱温为25℃,流动相为甲醇(A)-体积分数为2%的醋酸水溶液(B)梯度洗脱,流速为1.0ml/min,检测波长为321nm。结果:阿魏酸和欧前胡素分别在质量浓度为0.012~1.92μg、0.01~0.64μg内与峰面积呈良好的线性关系(r=0.9963~0.9959);平均加样回收率和RSD分别为98.28%、101.04%和2.87%、2.53%。结论:该方法可用于九味羌活丸的质量控制。
Objective：To establish RP-HPLC method for the determination of ferulie acid and imperatorin. Methods：The I-IPLC separation was achieved on a C 18 （4.6 mm×150 mm,5μm） at 25 ℃ .The mobile phase consisted mothanol-2% glacial acetic acid solution with gradient elution at a flow rate of 1.0 ml/min. The detection wavelength was set at 321nm. Results：The good linear correlation in the ranges of 0.012-1.921xg and 0.01-0.64μg（ r=0.9963-0.9959）for ferulic acid and imperatorin .The average recovery rates and RSD were 98.28%, 101.04% and 2.87% ,2.53%, respectively. Conclusion： The method could be used for quality control of Jiuwei Qianghuo pills.
作者
史雪霞
李运
Shi Xuexia Li Yun(The Affiliated Hospital of Qinghai University, Xining 810001, China Lanzhou Institutes for Food and Drug Control,Lanzhou 730030, China)
出处
《甘肃医药》
2017年第7期516-518,527,共4页
Gansu Medical Journal
基金
兰州市科技计划项目(项目编号:2016-3-17)
