摘要
目的了解广州海珠区霍乱病原体O1/O139群霍乱弧菌的流行、分布状况及生物学性状,为霍乱疫情的有效防控提供科学依据。方法对2002年-2016年本区肠道门诊腹泻患者、患者接触者等疫点处理、珠江河水、饮用水、养殖水、各种水产品、市售熟食、健康人群等标本进行O1/O139群霍乱弧菌的分离培养、形态鉴别、生化鉴定及血清分型,对检出的O1群霍乱弧菌进行噬菌体-生物分型,并对检出的部分菌株进行ctx A毒力基因及tcp A毒力基因检测。结果13 385份标本中O1/O139群霍乱弧菌阳性的有84份,阳性率为0.63%。84株菌株中,O1群67株,构成比为79.8%,分属于16个不同的噬菌体-生物型,小川2f型为优势;O139群17株,构成比为20.2%。从患者分离的流行株携带ctx A毒力基因,非流行株与O139群菌株未发现携带ctx A毒力基因。结论小川型、稻叶型及O139群霍乱弧菌在本地区并存,且其传播和流行呈逐渐减弱的趋势;流行株携带ctx A毒力基因,可引发较大规模的疫情暴发,而更多的散发则是由非流行株或O139群霍乱弧菌所引发。
Objective To study the epidemic condition,distribution and biological characteristics of O1/O139 Vibrio cholerae（ V. cholerae） in Haizhu district,so as to provide scientific basis for the prevention and control of cholera. Methods Stool or swabs samples of diarrhea patients and epidemic spots,water from Zhujiang river,drinking water,aquaculture water,aquatic products,delicatessen foods and healthy crowd samples were conducted fro the isolation culture,morphological identification,biochemical identification and serotyping of O1/O139 V. cholerae from 2002 to 2016. Some detected strains were conducted for the detection of ctx A and tcp A virulence gene. Results 13 385 samples were collected and O1/O139 V. cholerae was found in 84 samples,and positive rate was 0. 63%. Serotyping of the 84 V. cholerae strains showed that there were 67 O1 V. cholerae strains（ 79. 8%）,which belonged to 16 different phagebiological types and Ogawa type 2f was dominant; the rest 17 strains were O139 V. cholerae（ 20. 2%）. The strains isolated from patients carry ctx A gene,non epidemic strains and O139 strains didn＇t carry ctx A virulence gene. Conclusion Ogawa strains,inaba strains and O139 strains V. cholerae co-existed in this region,and its spread and popular showed a trend of gradual decreasing. Epidemic strains carry ctx A virulence genes,which maybe trigger a large-scale outbreak. And more sporadic cases were caused by the non-epidemic strains or O139 V. cholerae.
出处
《中国卫生检验杂志》
CAS
2017年第14期2109-2112,共4页
Chinese Journal of Health Laboratory Technology
