摘要
目的观察大鼠心律失常后缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)和心肌血管内皮生长因子-A(vascular endothelial growth factor-A,VEGF-A)的表达变化,探讨心律失常与冠状动脉供血不足引起的急性心肌缺血中两指标表达规律的不同。方法利用CaCl_2诱导大鼠心律失常,应用免疫组织化学染色、Western印迹法和实时荧光定量PCR检测大鼠心律失常后6 h内HIF-1α和VEGF-A的表达及变化情况。结果心律失常致死的大鼠心肌组织中HIF-1α及VEGF-A呈弥漫性表达,在心律失常发生的早期两者表达均呈增加随后下降;心律失常发生时即产生广泛的心肌缺血且范围不随时间增大。结论HIF-1α和VEGF-A在心律失常大鼠心肌组织中均有表达,可为致死性心律失常和冠状动脉供血不足引起的急性心肌缺血的鉴别诊断提供依据。
Objective To observe the expression changes of hypoxia inducible factor-1α（HIF-1α） and vascular endothelial growth factor-A（VEGF-A） in rats with arrhythmias, and to explore the differences of the expression pattern in the two indicators of acute myocardial ischemia caused by arrhythmias and coronary insufficiency. Methods The arrhythmia was induced by Ca Cl2, and the expression changes of HIF-1α and VEGF-A were detected by immunohistochemistry, Western blotting and real-time PCR within 6 h after the arrhythmia in rats. Results The expression of HIF-1α and VEGF-A showed diffuse in the myocardial tissue of rats died from arrhythmias. Both of them increased in the early arrhythmia,then decreased. Extensive myocardial ischemia happened at the beginning of arrhythmia occurrence and its range didn＇t expand with time. Conclusion The expressions of HIF-1α and VEGF-A in myocardium of the rats with arrhythmia can provide evidence for the differential diagnosis of acute myocardial ischemia caused by fatal arrhythmia and coronary insufficiency.
出处
《法医学杂志》
CAS
CSCD
2017年第3期225-231,共7页
Journal of Forensic Medicine
基金
国家自然科学基金资助项目(81273343)
关键词
法医病理学
心律失常
心性
心肌
缺氧诱导因子1
血管内皮生长因子-A
大鼠
forensic pathology
arrhythmias
cardiac
myocardium
hypoxia inducible factor 1
vascular endothelial growth factor-A
rats
