摘要
目的研究聚乙二醇干扰素α-2a(polyethylene glycol interferonα-2a,PEG-IFNα-2a)对慢性乙型肝炎(chronic hepatitis B,CHB)患者外周血中干扰素-γ(interferon-gamma,IFN-γ)及γ干扰素受体1(interferon gamma receptor 1,IFNGR1)水平的影响.方法应用PEG-IFNα-2a治疗的不同时间点抽取CHB患者及健康对照组静脉血,酶联免疫吸附法检测其血清IFN-γ浓度,实时荧光定量RT-PCR技术检测其外周血单个核细胞(peripheral blood mononuelear cells,PBMCs)表面IFNGR1的表达情况.结果 CHB组患者IFN-γ及IFNGR1均高于健康对照组(P<0.05).在使用聚乙二醇干扰素抗病毒治疗过程中,CHB患者IFN-γ及IFNGR1在12 wk时达到高峰,此后开始下降,至48 wk时低于治疗前水平,但仍高于健康对照组(P<0.05).抗病毒治疗12 wk时,乙型肝炎病毒(hepatitis B virus,HBV)DNA阴转组IFNGR1的表达水平高于HBV DNA未阴转组(2.22±0.65 vs 1.35±0.71),两者差异有统计学意义(P<0.05).结论 IFN-γ参与了CHB的发病,治疗12 wk时患者PBMCs表面IFNGR1的表达水平可能作为干扰素抗HBV治疗早期预测干扰素疗效的因子.
AIM To detect serum levels of interferon-γ, （IFN-γ） and expression of gamma receptor I （IFNGR1） on peripheral blood mononuclear cells （PBMCs） in patients with chronic hepatitis B （CHB） after treatment with polyethylene glycol interferon α-2a （PEG-IFN（α-2a）. METHODS Serum levels of IFN-γ were determined using ELISA in patients with chronic hepatitis B and healthy controls, and the expression of IFNGR1 on PBMCs was determined using real-time fluorescent quantitative RT-PCR. RESULTS Serum levels of IFN-γ and exprssion of IFNGR1 on PBMCs in patients with chronic hepatitis B were higher than those in healthy controls （P 〈 0.05）. IFN-γ, and exprssion of IFNGR1 on PBMCs in patients with chronic hepatitis B peaked at 12 wk after treatment with PEG-IFNα-2a, and then began to decrease, which was lower than pre-treatment at 48 wk, but still higher than that of healthy controls （P 〈 0.05）. The expression of IFNGR1 on PBMCs in the hepatitis B virus （HBV） DNA negative group was higher than that in the HBV DNA positive group （2.22 ± 0.65 vs 1.35± 0.71, P 〈 0.05） at 12 wk after treatment with PEG-IFNα-2a. CONCLUSION IFN-γ may participate in the pathogenesis of chronic hepatitis B, and the expression of IFNGR1 on PBMCs in patients with chronic hepatitis B at 12 wk of treatment may be used as an early predictor of interferon efficacy.
作者
程笑
汪莉萍
韩方正
庞亚博
黄姜伟
Xiao Cheng Li-Ping Wang Fang-Zheng Han Ya- Bo Pang Jiang-Wei Huang(Department of Infectious Diseases, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China)
出处
《世界华人消化杂志》
CAS
2017年第19期1743-1749,共7页
World Chinese Journal of Digestology
