摘要
目的探讨JNK抑制剂对烧伤后大鼠肠黏膜损伤的干预作用及其可能的机制。方法将72只SD大鼠,分为正常对照(C)组8只,烧伤对照(B)组32只,烧伤+SP600125干预(A)组32只。A组与B组给予90℃水烫伤7S,造成30%体表面积Ⅲ度烧伤。A组于烧伤前2小时腹腔注射SP600125,剂量20 mg/kg。A组、B组烧伤后第1天、第3天、第5天、第7天各组分别处死大鼠8只,C组大鼠实验后第2天处死。采用HE染色法观察烧伤后大鼠回肠组织形态学变化;通过流式细胞仪检测大鼠回肠凋亡率的变化;采用酶联免疫吸附(ELISA)法检测大鼠回肠中细胞因子IL-6和TNF-a的含量;免疫组织化学染色检测p-JNK表达。结果与B组相比,A组各时相点回肠黏膜凋亡率均减少,IL-6和TNF-a含量降低,p-JNK表达减少,差异有统计学意义(P<0.05)。结论通过抑制p-JNK表达,可以减少烧伤模型大鼠回肠黏膜组织细胞凋亡,降低炎性介质IL-6和TNF-a含量,从而减轻黏膜组织损伤。
Objective To investigate the intervention effect of JNK inhibitors on intestinal mucosa injury in rats after burn and its possible mechanism. Methods 72 SD rats were divided into normal control（C） group 8, burn control（B） group 32, and burn ＋SP600125 intervention（A） group 32. A group and B group were treated with 90 degree water burn 7S, resulting in 30% surface area, third degree burns. Group A received intraperitoneal injection of SP600125 2 hours before burn, with a dose of 20 mg/kg. Rats in group A and group B were sacrificed first days, third days, fifth days and seventh days respectively. 8 rats were killed in each group, and C rats were sacrificed second days after the experiment. HE staining method was used to observe the morphological changes of the ileum in rats after burn; The apoptosis rate of ileum in rats was measured by flow cytometry; Enzyme linked immunosorbent assay（ELISA） was used to detect the levels of cytokines IL-6 and TNF-a in the ileum of rats; Immunohistochemical staining was used to detect the expression of p-JNK. Results Compared with group B, the apoptotic rate of ileum mucosa in A group was decreased, the content of IL-6 and TNF-a decreased, and the expression of p-JNK decreased, the difference was statistically significant（P〈0.05）. Conclusion By inhibiting the expression of p-JNK, it can reduce the apoptosis of the ileum mucosa tissue and reduce the content of inflammatory mediators IL-6 and TNF-a in the burn model rats, thereby reducing the damage of mucosal tissue.
出处
《中国继续医学教育》
2017年第16期208-210,共3页
China Continuing Medical Education
