摘要
本研究利用组织块培养法从健康的银杏根、茎中分离得到116株内生真菌。通过显色反应、高效液相色谱(HPLC)等方法对内生真菌的代谢产物进行分析,最终筛选出2株能够产黄酮的内生真菌,利用分光光度法测定其发酵液中总黄酮产量均达到20 mg/L以上。形态学特征和真菌r DNA间隔序列(Internal Transcribed Spacer,ITS)的系统进化分析结果表明两株菌分别属于青霉属和毛霉属,其中青霉属为产黄酮银杏内生真菌的首次报道。本研究为利用微生物发酵生产黄酮类化合物奠定了良好的基础。
Many studies have shown that endophytes can co-evolution with host, and can produce similar or identical physiologically active substances as host. Flavonoid is one of most active compounds in extracts of Ginkgo biloba. In the present study,the method of tissue culturing was used to isolate endophytic fungi from health ginkgo roots and stems. After repeated separation and purification, 116 Ginkgo endophytes were isolated. Metabolites of these fungi was preliminarily analyzed by color reaction and high performance liquid chromatography （HPLC）, and two flavonoids producing endophytes were finally screened out. The production of flavonoids by these two strains were all more than 20 mg/L. These two endophytic fungi strain were identified as Mucor spp. and PeniciUium spp. by morphology characteristics and Internal Transcribed Spacer （ITS） sequences analysis, and PeniciUium spp. was the first report in the flavonoid producing endophytic fungus from G. biloba. This study laid a good foundation for the production of flavonoids by microbial fermentation, and these two strains can be induced to improve the production of flavonoids by mutation breeding before they are used to produce flavonoids by fermentation.
作者
何福林
陈小明
张瑞
吴月
袁志辉
HE Fu-lin CHEN Xiao-ming ZHANG Rui WU Yue YUAN Zhi-hui(College of Chemistry and Bioengineering in Hunan University of Science and Engineering Hunan Provincial Engineering Research Center for Ginkgo biloba Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South Hunan Hengwei Pharmaceutical Co. ,LTD , Yongzhou 425199, China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第7期1141-1147,共7页
Natural Product Research and Development
基金
湖南省教育厅科研项目(15C0585)
湘南优势植物资源综合利用湖南省重点实验室开放基金项目(XNZW15C11)
湖南省大学生研究性学习和创新性实验计划项目(2016-283)
关键词
银杏
内生真菌
黄酮类化合物
鉴定
Ginkgo biloba
endophytes
flavones
identification
