摘要
目的:观察补肾壮筋汤对腰椎间盘退变大鼠椎间盘及行为学特征的影响,并观察其安全性。方法:选取40只清洁级8周龄雌性SD大鼠,随机分为假手术组、模型组、补肾壮筋汤组、吲哚美辛组,每组10只。应用椎间盘穿刺法对模型组、补肾壮筋汤组、吲哚美辛组大鼠L5~6椎间盘进行椎间盘退变造模,假手术组充分暴露L5~6椎间盘前缘,不进行穿刺干预。造模后24 h,补肾壮筋汤组大鼠以补肾壮筋汤(1.5 g·kg^(-1))灌胃,吲哚美辛组大鼠以吲哚美辛胶囊水溶液(7.5 mg·kg^(-1))灌胃,假手术组、模型组大鼠均以生理盐水(10 mL·kg^(-1))灌胃,每天1次,连续给药7周。分别于造模前及药物干预开始后1、3、5、7周时进行机械痛阈实验和斜板实验。最后一次行为学特征测定结束后,从眼底静脉丛取血测定血清丙氨酸氨基转移酶(alanine aminotransferase,AAT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、肌酐(creatinine,CRE)、尿素氮(urea nitrogen,UN)的水平;取L5~6椎间盘、肝脏及肾脏,椎间盘组织分别进行HE染色、改良番红O-固绿染色后观察组织形态变化,肝脏及肾脏组织进行HE染色、Masson染色后观察组织病理改变。结果:(1)机械痛阈实验痛阈值。造模前及药物干预开始后不同时点痛阈值的差异有统计学意义,即存在时间效应(F=36.245,P=0.000)。假手术组痛阈值随时间变化趋势不明显;模型组痛阈值随时间变化逐渐降低;补肾壮筋汤组和吲哚美辛组的痛阈值于药物干预开始后1周时降至最低,后期变化不明显。4组大鼠痛阈值总体比较,差异有统计学意义,即存在分组效应(F=54.170,P=0.000)。除造模前外(F=1.375,P=0.266),药物干预开始后各时点4组大鼠痛阈值比较,组间差异均有统计学意义(F=30.059,P=0.000;F=34.117,P=0.000;F=33.454,P=0.000;F=35.617,P=0.000);药物干预开始后各时点假手术组痛阈值均高于模型组(P=0.000;P=0.000;P=0.000;P=0.000);除药
Objective：To observe the effect of Bushen Zhuangjin Tang（补肾壮筋汤,BSZJT） on intervertebral disc and behavioral characteristics of rats with lumbar intervertebral disc degeneration and its safety.Methods：Forty 8-week-old clean-grade female SD rats were selected and randomly divided into sham-operated group,model group,BSZJT group and indometacin group,10 cases in each group.The models of L5/L6 intervertebral disc degeneration（IVDD） were built in rats in model group,BSZJT group and indometacin group by using intervertebral disc puncture method,while the rats in sham-operated group were treated with sham-operation to adequately expose the anterior border of L5/L6 intervertebral disc without any puncture intervention.At 24 hours after the modeling,the rats in BSZJT group and indometacin group were intragastric administrated with BSZJT（1.5 g/kg） and indometacin solution（7.5 mg/kg） respectively,while the rats in sham-operated group and model group were intragastric administrated with normal saline（10 m L·kg^-1）,once a day for consecutive 7weeks.The mechanical paw withdrawal threshold（MPWT） test and the tiltboard test were carried out before modeling and at 1,3,5 and 7 weeks after the beginning of drug intervention respectively.The blood were fetched out from fundus oculi venous plexus after the last behavioral characteristics measurement,and the serum levels of alanine aminotransferase（AAT）,aspartate aminotransferase（AST）,creatinine（CRE） and urea nitrogen（UN） were measured.The L5/L6 intervertebral discs,livers and kidneys were fetched out.The intervertebral disc tissues were received HE staining and improved safranin O-fast green staining respectively and the hepatic tissues and nephridial tissues were received HE staining and Masson staining for observing the histopathological changes.Results：There was statistical difference in the pain threshold values between different timepoints before modeling and after the beginning of drug intervention,in other words,there wa
作者
赵赫
高誉珊
胡振国
陈思学
刘涛
杨永栋
俞兴
ZHAO He GAO Yushan HU Zhenguo CHEN Sixue LIU Tao YANG Yongdong YU Xing(Dongzhimen hospital of Beijing University of Traditional Chinese Medicine, Beijing 101121, China Beijing University of Traditional Chinese Medicine, Beijing 100029, China)
出处
《中医正骨》
2017年第7期12-20,26,共10页
The Journal of Traditional Chinese Orthopedics and Traumatology
