摘要
为了解新疆某医院粪便样本中人源性隐孢子虫种系基因型,将收集来的腹泻病人粪便样本采用乙酸乙酯-改良抗酸染色法进行卵囊鉴定,同时提取隐孢子虫感染阳性样本的核酸,设计特异性引物扩增隐孢子虫的18 S rRNA基因和HSP70基因,依据所获得的目的基因序列构建系统发生分析。结果表明,新疆地区人粪便中隐孢子虫的阳性率为16.5%,高于全国平均水平。系统进化分析显示,其分子生物学特征主要为微小隐孢子虫(C.parvum)和人隐孢子虫(C.hominis)。说明新疆地区人源性隐孢子虫种系主要为C.parvum及C.hominis,具备人兽共患传播的可能性。
To investigate the genetic diversity of human Cryptosporidium in Xinjiang region, an initial screening of Cryptosporidium from human fecal specimens with diarrhea symptom was performed by morphological identification with ethyl acetate-modified acid fast staining method. The 18 S rRNA and HSP70 genes of Cryptosporidium were amplified,and phylogenetic analyses were performed using data sets comprising the two genes from each positive sample. The results showed that samples collected from the hospital had much higher Cryptosporidiurn-positive rates than the national average, with prevalence values of 16.5 %. Phylogenetic analyses revealed the main species of Cryptosporidium found in Xinjiang were C.parrum and C.horninis. Therefore,the Cryptosporidium circulating in Xinjiang may be served as the important zoonotic pathogens.
出处
《动物医学进展》
北大核心
2017年第8期53-56,共4页
Progress In Veterinary Medicine
基金
新疆维吾尔自治区自然科学基金项目(2012211B13)
