摘要
以人工饲料中添加低浓度的Cry1Ac杀虫蛋白(0、5、10、20μg/g)饲养甜菜夜蛾5个世代,探讨低浓度杀虫蛋白连续刺激对该虫处女蛾与产卵蛾飞行速度、飞行时间与飞行距离的影响。结果表明,甜菜夜蛾对低浓度Bt毒素的响应经过了敏感期、逐步适应期到最终适应甚至反弹几个阶段:在F1代,对照组的飞行距离显著长于最高浓度处理组。F2、F3代,低浓度处理(5~10μg/g)后的甜菜夜蛾飞行力开始增强,高浓度杀虫蛋白处理组(20μg/g)的飞行力仍低于对照,不同处理在飞行时间与飞行距离上差异显著。在F4代,处女蛾的飞行力开始回升,特别是低浓度处理(5μg/g)的飞行时间和飞行距离显著高于对照,也显著或极显著高于其他2个浓度处理,各处理在飞行时间和飞行距离上达到显著差异。到了F5代,所有处理的飞行力均大于对照,其飞行速度、飞行时间与飞行距离3个指标在各处理间均达到显著差异。研究同时发现,无论是处女蛾,还是产卵雌蛾,对低浓度Cry1Ac杀虫蛋白的响应基本一致。文章认为,阐述毒蛋白对昆虫飞行的影响需要研究多个世代。
The effects of low concentration of Bt toxin on flight speed,flight time and flight distance of virgin and egg-laying adults were examined by adding Bt toxin to the artificial diets（0,5,10 and20μg/g）and feeding larvae with different diets for 5 generations.The results showed that,in F1 generation,the flight distance of adult in CK group was significantly longer than in20μg/g treatment;in the F2 and F3 generations,flight performance was enhanced after fed with low concentration of Bt toxin（5-10μg/g）,while high concentration of Bt toxin（20μg/g）inhibited flight of adults.In addition,there were significant differences in flight time and flight distance among different treatments.In F4 generation,flight ability of virgin adult started to increase,especially for the treatment with low concentration of Bt toxin（5μg/g）,the flight time and flight distance were significantly higher than those of the control,and significantly and extremely significantly higher than those of the other two treatments.There were significant difference in flight time and flight distance among different treatments.In F5 generation,after Bt treatments flight ability were all stronger compared to the control,and there were significant differences in flight speed,flight time and flight distance among all treatments.In this research,virgin and egglaying females both had similar responses to low concentration of Bt toxin.The study indicates that exact influences of Bt toxin on insect flight should be investigated for multi-generations.
作者
蔡文宸
苏宏华
陈玲
周浩
杨益众
Cai Wenchen Su Honghua Chen Ling Zhou Hao Yang Yizhong(College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Chin)
出处
《植物保护》
CAS
CSCD
北大核心
2017年第4期66-69,109,共5页
Plant Protection
基金
转基因生物新品种培育重大专项(2014ZX08012004
2016ZX08012004-010)
