摘要
目的观察癫痫清颗粒对戊四唑诱发大鼠慢性癫痫模型的影响。方法大鼠腹腔注射10 g·L-1戊四唑(40 mg·kg-1),隔日1次,连续4周,停药l周后,再次注射相同剂量的戊四唑,根据癫痫发作级别随机分为7组每组10只,连续灌胃给药治疗四周,末次给药1 h后除空白对照组外各组大鼠腹腔注射10 g·L-1戊四唑(40 mg·kg-1),记录各组大鼠癫痫发作级别,并通过HE染色观察癫痫清颗粒对戊四唑诱发大鼠慢性癫痫模型海马组织结构的影响,并通过免疫组化染色及免疫印迹法观察癫痫清颗粒对戊四唑诱发大鼠慢性癫痫模型海马组织凋亡因子表达的影响。结果癫痫清颗粒组癫痫发作级别显著低于模型对照组,与模型对照组相比癫痫清颗粒可显著降低大鼠脑海马CA1区AIF和Ba X的表达,与模型对照组相比癫痫清颗粒可显著增加大鼠脑海马CA1区Bcl-X/S+L的表达。结论癫痫清颗粒对戊四唑诱发大鼠慢性癫痫模型具有呈剂量依赖性的保护作用。
Objective This study was to investigate the effects of DXQ on pentylenetetrazole-induced hippocampal pyramidal cells apoptosis and cerebrum damage, then to explore its possible mechanisms. Method Male Wistar rats were randomly divided into 7 groups： normal control （NC）, pentylenetetrazole （PTZ）, Dian-Xian-Qing granule （DHQ） （4.78, 9.46, 18.92 g/kg· d ^- 1 ） , Dian-Xian-Ning Tablets （ DXN, 5.05 g/kg· d ^- 1 ） and Phenytoin Sodium Tablets （PST, 54 mg/kg· d ^- 1 ） positive control groups, a sub-convulsive dose of PTZ 40 mg/kg in the volume of 1 mL/kg（ip）was administered to rats on every second day for 30 days resulting in a permanent change of generalized tonic-clonic convulsions. The convulsive behaviors were evaluated according to Racine＇s score, the number of surviving hippocampal pyramidal cells was assessed by HE staining. The expression of AIF （apoptosis inducing factor） , BaX and Bcl-x/s ＋ L were analyzed by Western blot immunohistochemical （IHC） staining. Result Rats suffered PTZ showed higher Raeine＇s score, worse cell damage and apoptosis, which were all attenuated by DHQ （4.78, 9.46,18.92 g/kg-d-l）. Moreover, DHQ reversed the expression of AIF, BaX and Bel-x/s ＋ L induced by tonic-elonic convulsion. Conclusion These result suggest that DHQ has neuroprotective effects, which is related to the level of AIF, BaX and Bcl-x/s ＋ L restraining cells apoptosis.
出处
《实验动物科学》
2017年第3期10-15,共6页
Laboratory Animal Science
基金
国家科技部"十二五""重大新药创制"专项课题(No.2012ZX09102201-005)
辽宁省科技厅基金资助项目(No.2004226010-6)
