摘要
目的:分析不同病理分型鼻息肉中HIF-1α、VEGF和miR-200a表达及其与复发相关性研究。方法:选取鼻息肉患者42例,在随访期间有15例鼻息肉复发。采用免疫组化SABC法检测鼻息肉及下鼻甲组织中HIF-1α、VEGF表达水平,采用q RT-PCR技术检测鼻息肉及下鼻甲粘膜组织中miRNA-200a表达量。对比分析不同病理分型HIF-1α、VEGF、miRNA-200a表达差异并分析鼻息肉复发的因素。结果:鼻息肉中miR-200a表达量明显低于下鼻甲粘膜组织(P<0.05)。鼻息肉中HIF-1α、VEGF表达量明显高于于下鼻甲粘膜组织(P<0.05)。鼻息肉病组织中miR-200a表达量明显低于单发鼻息肉、多发鼻息肉(P<0.05);鼻息肉病标本中HIF-1α、VEGF表达量明显高于单发鼻息肉、多发鼻息肉组织(P<0.05)。鼻息肉复发与鼻息肉的病理分型、HIF-1α、VEGF表达密切相关(P<0.05)。结论:鼻息肉增生和局部血管的生成密切相关,HIF-1α可能是同构调节miR-200a表达,来控制VEGF及血管生成的。
Objective：To analyze the expressions of HIF-1 α,VEGF and miR-200a in different pathological types of nasal polyps and its correlation with recurrence.Methods：The data of 42 patients with nasal polyps were selected,and 15 of them had recurrence during the follow-up period.The immunohistochemical SABC method was applied to detect expression levels of HIF-1 α and VEGF in nasal polyps and inferior turbinate tissues.The qRT-PCR technique was used to detect the microrRNA 200a quantity expression in nasal polyps and inferior turbinate mucosa tissues.Compare the differences of expressions of HIF-1α,VEGF and miRNA-200a in different pathological classifications and analyze the factors of recurrence of nasal polyps.Results：The miR-200a expression in nasal polyps was significantly lower than in the inferior turbinate mucosa tissue （P〈0.05）.The HIF-lα and VEGF expressions in nasal polyps were significantly higher than in inferior turbinate mucosa tissues （P〈0.05）.The miR-200a expression level in nasal polyposis tissues was obviously lower than in tissues of single nasal polyps and multiple nasal polyps （P〈0.05）.The expressions of HIF-1α and VEGF in nasal polyposis specimens were obviously higher than in tissues of single nasal polyps and multiple nasal polyps （P〈0.05）.The recurrence of nasal polyps was closely related to the nasal polyps pathological classifications and expressions of HIF-1 and VEGF （P〈0.05）.Conclusion：Nasal polyps hyperplasia had close relation with the local blood vessel formation.HIF-1α may control the VEGF and angiogenesis by homogeneously regulating the miR-200a expression.
出处
《现代生物医学进展》
CAS
2017年第21期4059-4062,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81460094)
