摘要
目的:通过观察补阳还五汤大鼠急性脊髓损伤后大脑神经元细胞凋亡及IL-17的表达的影响,探讨其神经保护机制。方法:SD大鼠24只,分成正常组、假手术组、SCI组、BYHWD组,通过在T3-T4横突间横断右侧半脊髓制备SCI模型,分别于手术前1d及术后1 d、1 w、4 w、8 w运动BBB评分评定大鼠后肢运动功能;术后8 w用Tunnel法检测神经细胞凋亡、免疫组化法检测大鼠神经细胞IL-17的表达。结果:BBB评分比较:SCI组、BYHWD组BBB评分明显低于正常组与假手术组,差异有统计学意义(P<0.01);BYHWD组在术后4 w、8 w两个时间点均高于SCI组,差异有统计学意义(P<0.05)。细胞凋亡率比较:SCI组与BYHWD组细胞凋亡率均高于正常组与假手术组,且差异有统计学意义(P<0.01)。BYHWD组凋亡率低于SCI组且差异有统计学意义(P<0.01)。IL-17阳性细胞率比较:SCI组、BYHWD组IL-17阳性细胞率均显著高于正常组与假手术组(P<0.01)。BYHWD组IL-17阳性细胞率显著低于SCI组,差异有统计学意义(P<0.01)。结论:SCI后大脑皮层神经元的凋亡可能与IL-17的表达增加有关,补阳还五汤可能通过下调IL-17的表达从而减少细胞凋亡。
Objective：To observe the effects of Buyang Huanwu Decoction on the apoptosis and expression of IL-17 in cortex neurons in rats after spinal cord injury,in order to explore the possible mechanisms of its neuro protection.Methods：24 SD rats were divided into normal group,sham operation group,SCI group and BYHWD group.SCI model was established in T3-T4 inter transverse the right half of spinal cord transaction.BBB score of rat hind limb motor function were assessed at 1 d before operation and 1 d,1 W,4 W,8 W after operation.After 8 W,Tunnel method was used to detect the apoptosis of nerve cells,and the immunohistochemical staining was used to detect the expression of IL-17 in nerve cells of rats.Results：The BBB scores in SCI group and BYHWD group were significantly lower than in the normal group and sham operation group,and the difference was statistically significant （P〈0.01）.The BBB scores at 4 W and 8 W in BYHWD group were higher than in SCI group,and the difference was statistically significant （P〈0.05）.The apoptosis rate of SCI group and BYHWD group was higher than that in the normal group and the sham operation group,and the difference was statistically significant （P〈0.01）.The apoptosis rate of BYHWD group was lower than that of SCI group and the difference was statistically significant （P〈0.01）.The rate ofIL-17 positive cells in SCI group and BYHWD group was significantly higher than in normal group and sham operation group （P〈0.01）.The rate of IL-17 positive cells in BYHWD group was significantly lower than in SCI group,and the dif-ference was statistically significant （P〈0.01）.Conclusion：The apoptosis in cortical neurons after SCI may be related with the increase of IL-17,and the BYHW Decoction may reduce apoptosis by inhibiting the expression ofIL-17.
出处
《现代生物医学进展》
CAS
2017年第21期4028-4031,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81102726)
湖南省高校科技创新团队感染性疾病中医药防治研究团队开放科研课题(Grxjb-18
Grxjb-14)
