摘要
目的设计并合成高抑制活性的小分子Bcl-2蛋白抑制剂。方法基于已报道的活性小分子,运用Autodock 4.2软件研究其与Bcl-2蛋白的作用模式,探索Bcl-2蛋白的关键位点并设计、合成一系列新型小分子抑制剂,并进行体外抗肿瘤活性的实验。结果设计合成了8个全新的小分子化合物,其结构经1HNMR和13CNMR确证。结论所合成的8个化合物均有明显的体外抗肿瘤活性,且大部分高于阳性对照紫杉醇。
OBJECTIVE To design and synthesize Bcl -2 protein inhibitors of high inhibitory activity. METHODS Based on the active molecules which had been reported, the mode of action with the Bel - 2 protein were researched using Autodock 4.2, and a series of new molecule inhibitors were designed and synthesized. The in vitro anti - tumor activity experiments were studied on Hela, B16, C26 and SW620 cells by MIT method. RESULTS Eight kinds of new small Bcl -2 protein inhibitors were synthesized. The structures of the compounds had been determined by 1HNMR and 13 CNMR. CONCLUSION The new compounds process good anti - tumor activity,and the activity is much higher than the positive control drug.
出处
《华西药学杂志》
CAS
CSCD
2017年第4期347-353,共7页
West China Journal of Pharmaceutical Sciences
