摘要
目的:探讨间歇有氧运动激活心肌梗死(Myocardial Infarction,MI)大鼠肾脏miR-21/SIRT1/NF-κB通路改善肾功能的作用。方法:3月龄雄性SD大鼠,随机分为假手术组(Sham)、心肌梗死组(MI)、心梗+间歇运动组(ME),每组12只。左冠状动脉前降支(Left Anterior Descending Coronary Artery,LAD)结扎建立MI模型,ME组在心梗1周后采用小动物跑台进行8周间歇训练。训练结束后测定各组大鼠心电图变化,采用紫外分光光度法测定尿酸和尿蛋白水平,免疫荧光染色法测定AQP2表达,ELISA法测定血清AVP和尿液AQP2,RT-q PCR检测肾脏AQP2、V2R和miR-21表达,Western Blot检测肾脏AQP2、SIRT1和乙酰化NF-κBp65蛋白表达。结果:心梗大鼠肾脏功能显著降低,肾脏AVP、V2R和AQP2表达增多,尿液AQP2表达增多,水潴留严重。间歇运动可有效改善心梗大鼠肾脏功能,降低肾脏AVP、V2R和AQP2表达,减少尿液AQP2水平,上调肾脏miR-21和SIRT1的表达,抑制NF-κB乙酰化水平;且发现肾脏miR-21表达水平与SIRT1表达呈显著正相关。结论:间歇有氧运动可显著提高心梗大鼠肾脏miR-21表达,激活miR-21/SIRT1/NF-κB通路,改善心梗大鼠肾脏功能。间歇有氧运动改善心梗大鼠肾脏功能与提高心梗大鼠肾脏miR-21表达,激活miR-21/SIRT1/NF-κB通路关系密切。运动干预micro RNAs表达研究与心肾保护效应,将为心梗及肾脏疾病患者运动康复手段筛选提供新思路。
Objective： To determine the effects of aerobic interval training （AIT） on the expressions of renal miR-21, SIRT1, Ac-NF-κB and AQP2 in rats with myocardial infarction （MI）. Methods： male Sprague Dawley rats were randomly divided into Sham-operated group （Sham）, Sedentary MI group （MI） and MI with AIT group （ME） （n=12）. The MI model was established by ligation the left anterior descending coronary artery （LAD）, and rats in ME were subjected to 8 weeks treadmill exercise training. After training, cardiaorenal function was evaluated. The localization of AQP2 expression was determined by immunofluorescence staining. The levels of serum AVP and urine AQP2 were assessed by ELISA. The expression of renal AQP2, V2R and miR-21 was examined by RT-qPCR. The expression of renal SIRT1, Ac-NF-κBp65 and NF-κBp65 protein was examined by western blotting. Results： MI increased the expression of renal AVP, V2R and AQP2 and the level of urine AQP2, and then increased water reabsorption, thereby resulted in renal dysfunction. Compared with MI group, AIT obviously improved renal function and ameliorated the expression of renal AVP, V2R and AQP2 and the level of urine AQP2. Meanwhile, AIT up-regulated the expression of renal miR-21 and SIRT1, and inhibited the acetylation of NF-κBp65 in kidney of rats with MI. miR-21 expression was positively related to the expression of SIRT 1. Conclusion： AIT up-regulates the expression of renal miR-21, activates miR-21/SIRT1/NF-κB signalling pathway, and then im- prove the renal function in rats after MI. The protective effect of AIT on renal function in MI rats was related to the increased expression of miR-2l and the activation of the miR-21/SIRT1/NF-κB signaling pathway.
作者
林琴琴
耿元文
田振军
LIN Qin-qin GENG Yuan-wen TIAN Zhen-jun(Yanshan University, Qinhuangdao 066004, China Shaanxi Normal University, Xi＇ an 710119, China.)
出处
《体育科学》
CSSCI
北大核心
2017年第7期44-49,82,共7页
China Sport Science
基金
国家自然科学基金项目(31300978)
