摘要
目的评价滇结香花不同提取部位对四氯化碳诱导小鼠肝损伤的影响。方法取昆明种小鼠110只,随机分为空白对照组,模型组,联苯双酯组(阳性对照组,0.15 g·kg^(-1))以及滇结香花石油醚提取部位、30%乙醇提取部位、60%乙醇提取部位、90%乙醇提取部位的低、高剂量组(给药量分别为0.1,0.3 g·kg^(-1)),各剂量组连续给药7 d,腹腔注射0.2%四氯化碳花生油溶液(10 mL·kg^(-1))建立急性肝损伤小鼠模型,眼眶取血,测定血清中ALT、AST、TBIL、TBA的含量变化;小鼠肝脏处理,测定肝组织匀浆GSH、MDA、SOD含量,观察四氯化碳诱导急性肝损伤小鼠肝组织病理学变化。结果滇结香花不同提取部位不同剂量组中,除石油醚部位低剂量组、90%乙醇提取部位低剂量组外,其他剂量组均可显著降低血清中ALT、AST、TBIL水平(P<0.01);除石油醚低、高剂量组外,其他剂量组均可降低血清TBA水平(P<0.05,P<0.01)。测定肝组织匀浆中各项指标,石油醚高剂量组和30%及60%乙醇提取部位低、高剂量组中,MDA水平明显降低,GSH、SOD水平显著升高(P<0.05,P<0.01);小鼠肝组织病理学变化,除石油醚低剂量组、30%乙醇提取部位低剂量组及90%乙醇提取部位低、高剂量组外,其他剂量组损伤均有明显缓解。结论滇结香花30%乙醇提取部位高剂量组及60%乙醇提取部位低、高剂量组具有保护肝组织的作用,缓解四氯化碳造成的肝损伤,并且60%乙醇提取部位其作用效果明显优于阳性对照组。其机制可能是通过阻断脂质过氧化反应,清除体内过量的自由基,保护细胞的正常代谢,维持细胞的稳定性,该研究为一种新的用药资源提供了一定的理论依据。
Objective To study the protective effect of different extracts from Edgeworthia gardneri Meissn on carbon tetrachloride-induced liver injury in mice. Methods One hundred and ten adult male Kunming mice were randomly divided into normal control group, model group, bifendate group （positive control group, 0.15 g·kg^-1） , petroleum ether extract low- and high-dose groups （0.1, 0.3g·kg^-1） , 30 % ethanol extract tow- and high-dose groups （0.1, 0.3g·kg^-1） , 60 % ethanol extract low- and high-dose groups （0.1, 0.3g·kg^-1） , 90 % ethanol extract low- and high-dose groups （0.1, 0.3g·kg^-1）. After being treated with correspondent drugs with different dosages continuously for 7 days, experimental mice were intraperitoneally injected 0.2 % carbon tetrachloride peanut oil solution（ 10 mL·kg^-1）to copy the acute liver injury model except control group, then the orbital blood was taken and biochemical methods were used to measure ALT, AST, TBIL and TBA levels in the serum. Contents of MDA, and activities of GSH and SOD in liver tissue homogenate were determined to observe the pathological changes of liver tissue in mice with acute liver injury. Results Compared with the model group, the Edgeworthia gardneri Meissn extracts of different polarity parts with different doses groups, except petroleum ether extract low-dose group and 90 % ethanol extract low-dose group, the other groups had obviously low serum ALT, AST, TBIL, TAB levels （P 〈 0.05, P 〈 0.01 ）. With all indexes determined in liver homogenate, petroleum ether extract high-dose group, 30 % ethanol extract high-dose group, 60 % ethanol extract low- and high-dose groups had lower hepatic MDA content （P 〈 0.05） ; GSH and SOD activities were significantly increased in these groups （P 〈 0.05, P 〈 0.01 ）. The results of pathological examination showed that, apart from petroleum ether extract low-dose group and 90 % ethanol extract low-dose group, pathological injuries in liver tissues of the other groups were relieved to va
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2017年第4期454-458,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
江西中医药大学校级课题(藏药绿萝花药效物质基础及质量标准研究)
关键词
滇结香花
不同提取部位
肝损伤
四氯化碳
保护作用
Edgeworthia gardneri Meissn
carbon tetrachloride
liver injury
CC14
protective effects
