摘要
目的观察整合素连接激酶（ILK）在百草枯（PQ）致大鼠肺纤维化中表达水平的变化，探讨其与PQ致肺纤维化的关系。方法将40只雄性SD大鼠随机分成对照组和PQ组，每组20只，PQ组腹腔注射PQ溶液，对照组腹腔注射等量生理盐水，分别于染毒后7、14、28、56d各组取5只大鼠于麻醉下游离肺组织，利用肺组织切片观察肺组织病理改变情况，测定肺组织羟脯氨酸含量和ILK mRNA和蛋白的表达情况。结果肺组织切片HE和Masson染色示，PQ染毒后7d肺组织充血、水肿、炎症细胞浸润，14d炎症减轻，逐渐出现纤维增生，28、56d肺泡结构紊乱、肺间质纤维细胞沉积，出现大量蓝染的胶原纤维。与同期对照组比较，PQ组各时点肺组织羟脯氨酸含量均升高，差异均有统计学意义（P〈0．05）。各时点PQ组肺组织ILK mRNA和蛋白的表达量均高于同期对照组，差异均有统计学意义（P〈0．05）。P0组ILK mRNA和蛋白的表达量均于7d开始升高，28d达到峰值，56dILK的表达量有所降低。结论ILK的表达量随着PQ致大鼠肺纤维化的进展过程逐渐增加，ILK可能是PQ致肺纤维化的关键分子靶标之一。
Objective To observe the expression of integrin-linked kinase on pulmonary fibrosis of paraquat （PQ） poisoning rats, and to discuss the relationship between ILK with pulmonary fibrosis induced by paraquat. Methods Forty male Sprague-Dawley （SD） rats were randomly divided into control group and paraquat group, 20 in each group; the PQ group rats were intraperitoneally injected PQ liquid （18 mg/kg）, and the control group rats were injected the same volume of saline ; 5 rats of these two groups were respectively sacrificed after 7, 14, 28, 56 days of PQ injection; according to the results of lung biopsy HE staining and Masson staining to observe the lung pathologic changes, measure the value of lung hydroxyproline and the expression of ILK. Results HE and Masson staining of lung pathological biopsy showed, the 7th day after PQ exposure lung tissue mostly had congestion, edema, inflammatory cells infiltration; the 14th inflammation reduced, fibrosis change appeared gradually; the 28th and 56th showed the lung tissue structure disorder and occurred apparent hydroproline with blue dye in pulmonary interstitium. Compared with control group in the same experiment period, the value of lung hydroxyproline in each experiment period of PQ group increased （P〈0.05）, and the value of lung hydroxyproline of PQ group rose with the increasing of the time of PQ poisoning. The expression of ILK mRNA and protein in each experiment period of PQ group was higher than the control group in the same experiment period （P〈0.05） ; ILK mRNA and protein of PQ group began to increase on 7 day phase, reached the highest on 28 day phase, and decreased on 56 day phase. Conclusion The expression of ILK mRNA and protein increased with the lung fibrosis progression of PQ poisoning rats, so ILK could be the key molecule target which induced pulmonary fibrosis of PQ poisoning.
作者
苏艺伟
李艳华
李峰
冯玉超
黄辉
韦拔雄
刘移民
Su Yiwei Li Yanhua Li Feng Feng Yuchao Huang Hui Wei Baxiong Liu Yimin(Guangzhou Prevention and Treatment Center for Occupational Disease, Guangzhou 510620, China)
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
2017年第5期362-365,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
广州市民生科技重大项目（2014Y2-00067）
广东省“十二五”医学重点专科、广州市医学重点专科建设项目,广州市职业健康与健康效应实验室建设项目（2014SY00020）
广州市“121人才梯队工程”后备人才项目
关键词
百草枯
肺纤维化
整合素连接激酶
Paraquat
Pulmonary fibrosis
Integrin-liuked kinase
