摘要
采用超声波技术辅助提取菠萝皮渣多糖,利用正交设计实验优化其提取工艺,并利用体外实验研究该多糖对羟自由基(·OH)、DPPH自由基(DPPH·)的清除能力及其还原能力,以评价其抗氧化活性。研究结果表明,超声波辅助提取最佳工艺参数为:料液比1∶50(m/v),时间40 min,温度60℃,功率为570 W,该条件下多糖的得率为2.38%;在本实验浓度范围(1.50~3.50 mg/m L)内,菠萝皮渣多糖对·OH的清除率随其浓度的增加而增加,但清除能力均低于同浓度VC,清除DPPH·自由基的IC_(50)为2.47 mg/m L,对Fe^(3+)的还原能力随多糖浓度的增加而增加。可见,超声波辅助提取工艺效果较好,能缩短提取时间,提高菠萝皮渣多糖的得率;体外抗氧化实验表明菠萝皮渣多糖具有一定的抗氧化活性。从菠萝皮渣中提取活性多糖,可以变废为宝,实现菠萝资源的高值化利用。
Using pineapple peel as materials, optimal process conditions of ultrasound-assisted extraction of polysaccharides and its antioxidant activities were studied. Based on the single factor experiment, orthogonal experiment was conducted to optimize the extraction process. The antioxidant activities in vitro were also evaluated in three systems with hydroxyl radical （ ·OH）scavenging capacity, DPPH free radical（ DPPH· ）scavenging capacity and reducing capacity.The optimum conditions of pineapple peel polysaccharides were determined as follows ： material to liquid ratio was 1 ： 50, extraction time was 40 min, extraction temperature was 60 ℃, ultrasound power was 60%.Under the optimal conditions, the extraction yield was 2.38 % .The scavenging rate on hydroxyl radical （ · OH ） of the polysaecharides from pineapple peel increased with the increase of polysaceharides concentration （1.5-3.5 mg/mL）and was lower than Vc at the same concentration. The polysaceharides from pineapple peel had good DPPH scavenging effect when compared with standard Vc and the IC50 on DPPH. was 2.47 mg/mL. The reducing capacity on Fe^3＋ increased with the increasing concentration.So,the polysaccharides can be extracted effectively by this method and the polysaccharides have good antioxidant activities.The extraction of active polysaccharides from pineapple peel residue can realize the high value utilization of pineapple resources.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第15期207-211,217,共6页
Science and Technology of Food Industry
基金
广东省公益研究与能力建设项目(2015A010107015)
岭南师范学院热带与南海资源协同创新中心项目(CIL1503)
岭南师范学院自然科学研究项目(YL1403)联合资助
关键词
菠萝皮渣
多糖
超声提取
抗氧化
Pineapple peel
polysaccharides
ultrasound- assisted extraction
antioxidant activities
