摘要
该文探讨了长链非编码RNA(long non-coding RNA,1ncRNA)VLDLR在胰腺癌组织及5株细胞系中的表达,并分析了其对细胞增殖和迁移能力的影响。通过定量反转录PCR(quantitative reverse transcription PCR,qRT-PCR)检测VLDLR在14例胰腺癌组织和对应癌旁组织及5株细胞系中的表达水平,并进一步利用RNA干扰(ribonucleic acid interference,RNAi)技术探讨VLDLR的生物学功能。CCK-8和克隆形成实验检测细胞增殖能力,Transwell实验检测细胞迁移能力,Western blot检测蛋白质表达。结果发现,14例胰腺癌组织中,VLDLR相对表达水平为4.49±5.85(P<0.05),VLDLR在5株胰腺癌细胞中差异性表达。与对照组相比,实验组细胞中VLDLR m RNA水平明显降低(t分别为32.43、19.02,P<0.05);细胞增殖、细胞克隆形成及迁移能力明显均降低(P<0.05),基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)和MMP-9蛋白质水平降低(P<0.05)。该研究结果表明,VLDLR在胰腺癌中高表达,下调VLDLR表达可降低胰腺癌细胞的增殖和迁移能力。
The aim of this paper is to investigate the expression profile of long non-coding RNA VLDLR in pancreatic cancer tissues and 5 different cell lines and the effect of VLDLR on the proliferation and migration of pancreatic cancer cell. Quantitative reverse transcription PCR（qRT-PCR） was performed to detect the relative expression of VLDLR in 14 cases of pancreatic cancer tissues and corresponding para-carcinoma tissues and five cell lines. The proliferation rates were examined by CCK-8 and colony formation assays. The migration rates were examined by Transwell assays. The relative level of the protein was detected by Western blot. The relative level of VLDLR m RNA in 14 cases of pancreatic cancer tissues was 4.49±5.85（P〈0.05）. The relative level of VLDLR m RNA were different in five pancreatic cancer cell lines. Compared with the control group, VLDLR m RNA level was decreased（t=32.43, 19.02, P〈0.05） and the ability of proliferation, cell colony-forming and migration were significantly inhibited in experiment group（P〈0.05）. The protein levels of matrix metalloproteinase-2（MMP-2） and MMP-9 were reduced（P〈0.05）. These results indicated that the expression of VLDLR was up-regulated in pancreatic cancer. Knockdown of VLDLR could decrease the proliferation and migration of pancreatic cancer cell.
作者
孙姚承
王大为
徐岷
周海浪
龚爱华
陈吉祥
Sun Yaocheng Wang Dawei Xu Min Zhou Hailang Gong Aihua Chen Jixiang(Department of General Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China School of Medicine, Jiangsu University, Zhenjiang 212013, China)
出处
《中国细胞生物学学报》
CAS
CSCD
2017年第6期739-744,共6页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:81472333
81372718
81672402)资助的课题~~
